Journal of Bioequivalence & Bioavailability

Determination of Cefditoren Pivoxil by high performance liquid chromatographic method in tablets

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

S. Anusha, M. Mathrusri Annapurna, S. V. S. Goutam and P.Suresh

Posters: J Bioequiv Availab

Abstract:

C efditoren pivoxil is a third-generation semi-synthetic cephalosporin antibiotic for oral administration. It is a prodrug which is hydrolyzed by esterases during absorption, and the drug is distributed in the circulating blood as active cefditoren. Cefditoren is used to treat uncomplicated skin and skin structure infections, community-acquired pneumonia, acute bacterial exacerbation of chronic bronchitis (ABECB), pharyngitis, and tonsillitis. Various methods are available in the literature for the determination of cefditoren pivoxil in human plasma using HPLC, UPLC and spectrophotometry but in the present work an isocratic RP-HPLC method was proposed for the determination of cefditoren pivoxil in pharmaceutical formulations. Chromatographic separation was achieved by using a C-18 (250mm ? 4.6mm i.d., 5 μm particle size) column of Shimadzu Model CBM-20A/20 Alite, equipped with SPD M20A prominence photodiode array detector, maintained at 25 ?C. Isocratic elution was performed using acetonitrile and water as mobile phase. The overall run time was 10 min. and the flow rate of the mobile phase was 1.2 mL/min. with UV detection at 218 nm. 20 μL of sample was injected into the HPLC system. Linearity was observed in the concentration range of 1.0?250 μg/mL (R 2 = 0.999) and the method was validated as per ICH guidelines. The RSD for intra-day and inter-day precision were found to be less than 2 %. The percentage recovery was in good agreement with the labeled amount in the pharmaceutical formulations and the method is simple, precise, accurate and robust for the determination of Cefditoren pivoxil