Journal of Microbial & Biochemical Technology

Detection of waterborne pathogenic bacteria using immunofluorescence technique

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Mihai Nita-Lazar, Irina Eugenia Lucaciu, Catalina Stoica and Alina Roxana Banciu

National Research and Development Institute for Industrial Ecology, Romania

Scientific Tracks Abstracts: J Microb Biochem Technol

Abstract:

The waterborne pathogenic bacteria, especially the enteric bacteria of human fecal origin have become currently a global public health issue. The detection and quantification of drinking water microorganisms are an essential part of any quality control or water safety management plan interconnected to enteric bacterial pathogens such as Salmonella spp., Shigella spp., Vibrio cholerae or to nonfecal bacterial pathogens such as Legionella spp. The standard methods of detecting waterborne pathogenic bacteria are time-consuming due to the growing step in a specific culture media, followed by isolation, microbiological and/or serological identification and in some cases followed by subspecific characterization. This study aimed to develop a faster, powerful, more sensitive and reproducible diagnostic tool to monitor a specific pathogen contamination in drinking water by specific antibodyantigen interactions. Three pathogenic bacteria such as Pseudomonas aeruginosa, Escherichia coli and Legionella spp. were detected by immunofluorescence technique with fluorochome tagged antibodies. Our results showed a good specificity of the antibodies in a very complex bacterial mixt as well as a starting detection level from 1 bacteria/ml. Overall, these techniques proved to be a reliable one, time-effective and sensitive for diagnosis and prevention of drinking water quality and waterborne bacterial disease.

Biography :

Mihai Nita-Lazar has a wide expertise in the fields of Microbiology, Molecular Biology and Biochemistry mainly in prestigious laboratories such as the Institute of Microbiology, ETH, Zurich, Switzerland, Department of Biochemistry and Cellular Biology of Stony Brook University, New York, SUA, Department of Cellular and Molecular Biology of Medical University Center from Boston, Massachusetts, SUA and the Department of Microbiology, University of Medicine, Baltimore, Maryland, SUA. He is currently working as Principal Investigator of Bioassay-Biological Analysis Laboratory of the National R&D Institute for Industrial Ecology-ECOIND. He has published more than 40 ISI papers, four books/chapters, h-index 10.