Journal of Bacteriology & Parasitology

Detection of plasmid-mediated quinolone resistance and blandm-1 genes among extended-spectrum β-lactamase producing Klebsiella pneumoniae in Iran

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Farzaneh Firoozeh1, Ehsaneh Shams1, Rezvan Moniri1 and Mohammad Zibaei2

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

ESBL-positive Klebsiella pneumoniae is often resistant to non-β-lactam antibiotics especially fluoroquinolones, and
rapid dissemination of these resistant strains has led to the increased utilization of carbapenems. The emergence of
carbapenemases producing isolates is an outcome of increased utilization of carbapenems. Of the carbapenemases, the New
Delhi metallo-β-lactamase-1 (NDM-1) was first described in a Klebsiella pneumoniae isolate and becoming increasingly
prevalent in many countries. The aim of present study was detection of plasmid-mediated quinolone resistance and blaNDM-1
genes among extended-spectrum β-lactamase producing Klebsiella pneumoniae in Kashan, Iran. During the study period from
April 2013 to April 2014, 185 K. pneumoniae isolates were tested for quinolone resistance and ESBL producing using the disk
diffusion method and double disk synergy (DDST) confirmatory test. ESBL-producing strains were further evaluated for the
blaCTX-M genes. The PCR method was used for the detection of plasmid-mediated quinolone resistance and blaNDM-1 genes
and the purified PCR products were sequenced. The Double Disc Synergy Test (DDST) confirmed 47% (n=87) of isolates
as ESBL-producing Klebsiella strains. Majority of which (80.5%) carried blaCTX-M genes and 23% were positive for blaNDM-1.
All of them and 89.7% were multi drug- resistant (MDR) and fluoroquinolone resistant respectively. Seventy-seven ESBL
producing K. pneumoniae isolates harbored PMQR genes, which mostly consisted of aac(6’)-Ib-cr (70.1%) and qnrB (46.0%),
followed by qnrS (5.7%). Among the 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried 2 and 3 different PMQR
genes, respectively. However, qnrA and qepA were not found in any isolate. Our results highlight high ESBL occurrence with
CTX-M type and high frequency of plasmid-mediated quinolone resistance genes among ESBL producing K. pneumoniae
isolates in our region. Also multidrug-resistant K. pneumoniae isolates harboring blaNDM-1 are emerging in Iran.