Journal of Bacteriology & Parasitology

Detection of Capillaria philippinensis coproantigen in clinical cases of capillariasis by enzyme-linked immunosorbent assay using polyclonal antibodies

4^th International Conference on Parasitology

September 01-02, 2017 | Prague, Czech Republic

Salma M Abdel-Rahman, Hanaa Y Bakir, Ragaa A Othman and Mervat M Ali

Assiut University, Egypt

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

The present study develop and evaluate an enzyme-linked immunosorbent assay (ELISA) to detect Capillaria philippinensis antigen in stool elutes using rat and rabbit polyclonal antibodies. The detection of C. philippinensis antigen in stool could be more valuable in diagnosis before irreparable damage. In this study, adult C. philippinensis worms were collected and used to immunize rats and rabbits to raise antibodies. Rat antibodies are further used as primary capture to coat ELISA plates and rabbit hyper immune serum used as secondary capture of antibodies. Advances in immuno-diagnosis have focused on detection of parasite antigens in host body fluid; these tests have an advantage over antibody detection because antigenemia implies recent and active infection. The assay detected the antigen in 100% of microscopically confirmed cases of capillariasis. Antigen was also detected in 75% of stool elutes from clinically suspected cases of capillariasis with no eggs in their stool. This ELISA will improve the diagnosis of Capillaria infections and will be useful in following patients after treatment.