Journal of Bacteriology & Parasitology

Detection of antigens leading protective immunity against Campylobacter jejuni and investigation of potentional application area

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Mahdi Marzi, Tulin Guven Gokmen, Melda Meral, Yildiz Kalayci and Fatih Koksal

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Campylobacter jejuni, colonizes especially on the gastrointestinal tract of the poultry and leads economic losses. Transmission
to human occurs by consumption of infected poultry products and cause gastrointestinal symptoms. Colonization in
poultry increase in relation with the age. Non-colonization occurs in the first 2-5 weeks so there may be the role of maternal
IgY for protective immunity in eggs. Therefore antigens inducing protective immune responses may be used in vaccine studies
and serologic diagnostic kits for human and poultry. Determining the reactivity of anti-C. jejuni IgY in local chicken eggs with
membrane antigens was aimed. Membrane proteins of C. jejuni isolated from tissue samples of I, III and V weeks old chickschickens
treated in the slaughter house of regional industrial type enterprises were extracted. After separation by SDS-PAGE,
immobilized to nitrocellulose membranes. IgY were extracted from 100 eggs taken randomly from domestic and industrial
enterprises. Proteins reacting with IgY were investigated by immunoblotting. Colonization in 17.5% of tissue samples was
observed. Proteins from 8-120 kdA on cell mebranes were showed. IgY in 68% of the egg was determined by SDS-PAGE and
ELISA. Reactive IgY with major 36 and 60 kDa proteins (93.3-100%) in 30 (44.1%) of egg extracts were detected. Anti-C. jejuni
IgY was determined in 14% and 46% respectively in eggs obtained from the industrial and domestic enterprices and identified
immune reactive with 36 and 60 kDa proteins. Therefore these antigens, can be used as target antigens for the vaccine and
diagnostic kit development.