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Design an alcohol biosensor based on alcohol dehydrogenase and modified electrode with cdo nanoparticles
International Conference and Exhibition on Nanotechnology & Nanomedicine
March 12-14, 2012 Omaha Marriott, USA
Masoud Negahdary, Ali Sar zaeim and Marziyeh Ajdary
Posters: J Nanomedic Nanotechnol
Abstract:
In this work, we design a new alcohol biosensor for determination of ethanol. A new aspect of this project is use of cadmium oxide nanoparticles for facile electrochemical interaction. The carbon paste electrode (CPE) was used as worker electrode.in addition; electrochemical measurement was done versus saturated calomel electrode (SCE). A voltammetric ethanol biosensor was constructed using alcohol dehydrogenase (ADH). The alcohol dehydrogenase was immobilized on the surface of a carbon paste electrode modified by cdo. The linear range of this biosensor for ethanol determination was from 10 to 480 μM. The biosensor was tested with very good results for ethanol determination in beverages. At the end of the 15 th day, the biosensor retained 78% of its initial activity. Keywords: biosensor, alcohol, alcohol dehydrogenase enzyme, cdo nanoparticles