Journal of Nanomedicine & Nanotechnology

Cytotoxic and genotoxic effects of Titanium dioxide nanoparticles in testicular cells of male Wistar rat

2^nd International Conference on Nanotek and Expo

December 3-5, 2012 DoubleTree by Hilton Philadelphia Center City, USA

Ramovatar Meena,Kajal Kesari, Kavindra Kumar Keseri and Paulraj R

Accepted Abstracts: J Nanomed Nanotechol

Abstract:

Serious concerns have been expressed about potential risks of engineered nanoparticles. Regulatory health risk assessment of such particles has become mandatory for the safe use of nanomaterials in consumer products and medicines; including the potential effects on reproduction and fertility are relevant for this risk evaluation. In this study, we examined effects of intravenously injected titanium dioxide nanoparticles (TiO 2 -NPs; 21 nm), with emphasis on reproductive cellular and genotoxicity. Animals were sacrificed after 7 days and testis was dissected and various biochemical and pathological parameters were performed. Antioxidant enzymes such as Catalase, glutathione peroxidase, and superoxide dismutase showed a significant decrease, while significant increase in lipid peroxidase was observed. EDXRF analysis of testis confirmed the bioaccumulation of TiO 2 -NPs in testicular cells. In TiO 2 -NPs treated animals various functional and pathological disorders, such as reduced sperm count, increase in caspase-3 (a biomarker of apoptosis), creatine kinase activity and cell apoptosis was observed. Moreover the testosterone activity was decreased significantly in a dose dependent manner. It is concluded that TiO 2 -NPs induce oxidative stress, which produce cytotoxic and genotoxic changes in sperms and affect the fertilizing potential of spermatozoa