Journal of Pharmacovigilance

Cytoprotective effect of Hypericum triquetrifolium turra. On cyclophosphamideâ??induced cardiotoxicity in rat

6^th Pharmacovigilance Congress

September 28-30, 2016 Toronto, Canada

Songul Cetik

Mardin Artuklu University

Posters & Accepted Abstracts: J Pharmacovigil

Abstract:

Cardiotoxicity is one of the limiting side effects of this commonly used anticancer agent and cardiotoxic effects of CP were found to be dose-related cardiac damage, morphologically defined necrosis, bleeding. Hypericum triquetrifolium Turra.(HT) is a phenolic component with antioxidative and anticarciogenic properties. This study aimed to investigate the possible protective effect of HT on CP-induced cardiotoxicity. Serum aspartat transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), malondialdehyde (MDA), creatine kinase-MB (CK-MB), total oxidant state (TOS), total antioxidant state (TAS), oxidative stress index (OSI) was examined. Furthermore, the cardiac tissues were analysed histologically. Albino rats (Wistar, 3-4 months old, male, weight 220 ± 20 g healthy) were randomly divided in 9 groups, each including seven animals: Group 1 (control) treated with 0,5ml saline; groups 2 treated with 150 mg/kg CP, respectively; group 3, 4 and 5 treated with 25, 50 and 100 mg/kg HT; groups 6 , 7 and 8 treated with 25, 50 or 100 mg/kg HT+CP, group 9 treated with 0,5ml - %0,2 DMSO. The results was analyzed bye One Way Analysis of Variance and Kruskal-Wallis One Way Analysis of Variance on Ranks Test. Levels of AST, ALT, LDH, MDA, CK-MB, TOS, OSI were found high only in the CP groups. GSH and TAS levels were found low in the only CP groups. There was a dose-dependent on the CP-induced cardiotoxicity. Hemorrhage, inflammatory cell infiltration and the separation of the muscle fibers in the heart tissue supported the biochemical data. With 25, 50, 100 mg/kg HT, there was an important decrease in the CP toxicity and reduced inflammation and lipid peroxidation in the heart tissue and increase of serum glutathione (GSH) and total antioxidant capacity (TAS) levels were found when HT was applied. Based on these findings, it could be proposed that HT was a strong candidate in preventing the CP-induced cardiotoxicity but further clinical studies should be done in order to verify its application on humans.

Biography :

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