Journal of Bacteriology & Parasitology

Cytochrome B gene amplification: A novel approach for diagnosis of theileriasis in cattle under field conditions

International Conference on Medical Parasitology and Zoology

October 17-19, 2016 Houston, USA

Muhammad Faiz Rasool

University of Veterinary and Animal Sciences, Pakistan

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Theileria are intra cellular protozoa transmitted by ticks causing disease in ruminants. In Arthropods (vectors) it remains in gut and salivary gland, in host Theileria infect both types of bloods cells i.e., RBCâ??s and WBCâ??s. Infective stage (sporozoites) is released during tick feeding, after infection one cell may contain 40-50000 sporozoites. Infection by a single tick may lead to severe disease and death depending upon the immune response. In present research study 100 cows both infected and carriers were sampled from cattle farms and small holder farmers from Lahore and Kasur districts. Blood Samples were analyzed microscopically by Giemsa stain and at molecular level by amplifying Cytochrome B gene. Sets of primers Cytob R and Cytob R were designed to specifically amplify two different regions of the T. annulata cytochrome B. Total DNA was extracted from samples with the help of TIANGEN DNA Extraction kit following the manufacturer instructions. 100 DNA samples were quantified by Spectrophotometry to evaluate the concentration and quantity of extracted DNA from the blood samples. Then PCR for cytochrome B was performed. Percentages of negative and positive samples were recorded and analyzed by applying Chi square method. , in current research intra erythrocytic bodies were found in 82 samples while PCR showed 20 positive samples. During research it was found that five blood samples that were observed negative by simple microscopy, they displayed positive result after polymerase chain reaction.

Biography :

Email: faiz.rasool@uvas.edu.pk