Journal of Vaccines & Vaccination

Construction of a recombinant vaccinia virus expressing E gene of Japanese encephalitis virus and immunogenicity in pigs

16^th Euro Global Summit and Expo on Vaccines & Vaccination

June 19-21, 2017 Paris, France

Hui-jun Lu

Changchun Veterinary Institute of CAAS, China

Posters & Accepted Abstracts: J Vaccines Vaccin

Abstract:

Japanese encephalitis virus (JEV) can infect humans and swine with a high incidence rate in Asia. Consequently, the development of a vaccine that can provide rapid protection with minimal risk is an important research goal. A recombinant vaccinia virus (rVVTK/E3LÎ?â??E) containing envelope protease coding regions of JEV Yunnan0901 was constructed in this study. With the Tiantan strain of vaccinia virus (E3LÎ?), an attenuated vaccine was constructed with TK gene deletion by homologous recombination. After screening and identification with a selectable marker, the recombinant vaccinia virus candidate vaccine was evaluated for its ability to induce humoral and cellular responses in pigs. The animals were vaccinated twice with a 21-day interval, and groups of animals were inoculated with the attenuated vaccine, vaccinia virus or phosphate buffered saline (PBS). All pigs vaccinated with rVVTK/E3LÎ?â??E developed specific anti-JEV antibodies and neutralizing antibodies. Splenocytes from pigs immunized with rVVTK/E3LÎ?â??E showed higher levels of T-lymphocyte proliferation, the greatest amounts of interferon-γ and interleukin (IL)-2, and moderate amounts of IL-4 and IL-10 in the presence of JEV. Vaccination with rVVTK/E3LÎ?â??E provided greater protection than that by the attenuated vaccine against JEV challenge. Moreover, pigs inoculated with the recombinant vaccine rVVTK/E3LÎ?â??E showed a lower virus load than those given the attenuated vaccine. Our findings indicated that rVVTK/E3LÎ?â??E might be an attractive candidate vaccine for preventing JEV infection.

Biography :

Email: huijun_lu@126.com