Journal of Microbial & Biochemical Technology

Composition and biological activities of the essential oil of Nigella sativa seeds isolated by accelerated microwave steam distillation with cryogenic grinding

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Farid Benkaci-Ali, Rym Akloul, Meriem Zerrouki and Gauthier Eppe

University of Sciences and Technologies Houari Boumediène, Algeria
University of Liège, Belgium

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

In this study, essential oil of Sahara Nigella sativa L. was extracted using a rapid extraction, the microwave steam distillation (MSD) and the cryogenic grinding (CG). Two procedures have been investigated, the MSD1 (seeds inside of oven apparatus) and MSD2 (seeds outside of oven apparatus). Forty-six compounds were identified and significant differences in quantities of the major constituents were observed, mainly were thymoquinone (CLG: 331.82-443.55 mg and CG: 272.95-413.57 mg/100g of seeds), p-cymene (CLG: 181.71-244.17 mg, CG: 369.80- 374.40 mg/100g of seeds), dehydro-sabina ketone (CLG: 24.60- 25.83 mg, GC: 44.02-50.69 mg/100g of seeds), carvacrol (CLG: 10.32-10.96 mg, CG: 3.91-12.67 mg/100g of seeds) and longifolene (CLG: 11.90-16.43 mg, CG: 12.72-19.58 mg/100g of seeds). Results showed that essential oils exhibit a good activity in each antioxidant system with a special attention for β-carotene bleaching test (IC50: 21 to 27 μg/ml) and reducing power (EC50: 9 to 14 μg/ml). The Nigella sativa essential oils exhibited higher antibacterial and antifungal activities varying according to technique extraction and grinding mode used, with a high effectiveness against Gram-positive bacteria with a diameter of inhibition zones growth ranging from 9.5 to 35 mm and MIC and MBC values ranging from (0.042â??0.10 mg/ml) to (0.20â??0.75 mg/ml), respectively.

Biography :

Benkaci-Ali Farid B.Sc. and M.Sc. degrees at the National Polytechnic School and Algerian Petroleum Institute. His PhD (2007) on green extraction techniques was realized with Baaliouamer and Michel Marlier Professors at University Houari Bouand mediene of Sciences and technology of Algiers USTHB and Faculty of Agronomy of Gembloux in Belgium. The transition to natural products was made as a result of a postdoctoral spell at . Laboratory of Mass Spectrometry, L.S.M, University of Liege working on the green method of extraction and new chromatographic analysis techniques and the impact of the extraction techniques on the antibacterial and antifungal properties with Professors Edwin De Pauw and Gauthier Eppe. He was appointed to an assistant lectureship at USTHB  in 2007. In 2008 he gained tenure and was appointed to a lectureship until he took the position of associate Professor in 2009.