Journal of Microbial & Biochemical Technology

Comparative study for the antibacterial activity of the Amla (Emblica officinalis) phenolic extract and some antibiotics against four pathogenic bacteria in vitro

International Conference and Summit on Industrial & Pharmaceutical Microbiology

October 17-18, 2016 Kuala Lumpur, Malaysia

Waffa A Ahmed, Ban N Nadhom and Narjes A Abdulrazaq

University of Baghdad, Iraq

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

This study was conducted on phytochemistry and antibacterial activity of Amla phenolic extract against Staphylococcus aureus, Proteus spp., Escherichia coli and Bacillus spp., these strains were obtained from zoonosis research unit of Veterinary Medicine College of Baghdad University. The Emblica officinalis phenolic extract was prepared from dried fruit of Emblica officinalis, Thin Layer Chromatography Analysis (TLC) screening of phytochemical analysis showed a content of Emblicanin compounds, Gallic and Ellagic acids, Quercetin, Chebulinic and Chebulagic acids antibacterial activity for phenolic extract was performed by well diffusion technique using Muller Hinton agar which were seeded with 0.1 ml of standardized inoculum of each tested organism with reference to Mcfarland turbidometry. Results showed antibacterial activity of Emblica officinalis extract against Staphaureus, Proteus spp., Escherichia coli and Bacillus spp., isolates at different concentrations. The highest inhibition zone for S. aureus bacteria ranged from 16±0.57 to 33.3±0.3 at 10 mg/ml towards 100 mg/ml concentrations respectively in comparison with inhibition zones which were recorded for Bacillus spp., 11.3±0.6 mm to 28±0.06 mm, Escherichia coli 10.6±1.2 to 22.6±1.2 mm and Proteus spp., 8.2±0.1 to 20.3±0.57 at same concentrations. The susceptibility of these isolates against antibiotics (Clindamycin 2 mcg, Imipenem 10 mcg, Vancomycin 30 mcg, Novobiocin 30 mcg, Trimethoprim 5 mcg, Erythromycin 15 mcg, Ampicillin 10 mcg, Streptomycin 10 mcg, Cefotaxime 30 mcg, Rifampin 5 mcg) was studied according to the species of strains. Results showed that these strains were differed in their susceptibility to antibiotics. E. coli isolates were resistant 100% to Ampicillin, Trimethoprim and susceptible 100% to Imipenem, while Proteus isolate were resistant 100% to Clindamycin, Erythromycin, 80% Vancomycin and susceptible to Cefotaxime, Trimethoprim. Staphylococcus spp., was 100% resistant to Cefotaxime, Streptomycin, Trimethoprim and 100% susceptible to Clindamycin, Vancomycin while Bacillus spp., isolates were 100% resistant to Streptomycin, Trimethoprim, Ampicillin and 100% susceptible to Imipenem and 80% to Clindamycin and Erythromycin. The results revealed that Emblica officinalis extract was more effective against all bacteria used in the current study and can be used to treat infections caused by these pathogens after suitable clinical trials.

Biography :

Email: waffaabduleah@yahoo.com