Journal of Microbial & Biochemical Technology

Comparative genomic analysis of genus Amycolatopsis: Revealing their phylogeny, functional attributes and potential to produce variety of secondary metabolites

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

Rashmi Kumari, Roshan Kumar, Priya Singh, Udita Mukherjee, Utkarsh Sood, Princy Hira and Rup Lal

University of Delhi, India

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

The genus Amycolatopsis comprises a large group of medicinally and commercially important Gram positive filamentous bacteria. They are prolific producers of a large number of biologically active compounds viz. antibiotics, anticancer agent, immune suppressant etc. Although genomes of species belonging to genus Amycolatopsis have been sequenced but these have not been analyzed. This study focus on the intra and inter-generic comparative genomic analysis of 24 Amycolatopsis strains with emphasis on phylogeny, functional attributes and secondary metabolite producing ability. Phylogenetic analysis based on 16S rRNA gene sequence, Average Nucleotide Identity and ribosomal genes were in consensus with each other and these strains clustered in four distinct groups based on the biosynthetic gene clusters they harbour. All strains of A. mediterranei were 99.9% similar and cluster together with A. rifamycinica (92.51%) and A. vancoresmycina (91.52%) as they also harbour the rif PKS cluster. Phylogeny revealed a major drawback in classification of four A. orientalis strains based on their ANI values and need to be reclassified in order to generate proper phylogenetic assessment of genus Amycolatopsis. Functional analysis revealed the two-component system for gene expression was highly abundant in all the strains along with ABC transporters. Detailed analysis led to construction of a novel rifamycin biosynthetic gene cluster in A. rifamycinica which differed by 10% at nucleotide level from rif biosynthetic gene cluster of Rifamycin B producer A. mediterranei S699. In conclusion, this study revealed the genetic key components of genus Amycolatopsis and provided an insight into secondary metabolite biosynthetic gene clusters across different species.

Biography :

Rashmi Kumari has obtained her Master’s Degree in Zoology from Department of Zoology, University of Delhi. Presently she is pursuing her Doctoral degree in Molecular Biology at Department of Zoology, University of Delhi. Her Doctoral research focuses on the Genetic Manipulation of rifamycin biosynthetic gene cluster of Amycolatopsis mediterranei S699 for production of rifamycin analogues.