Journal of Vaccines & Vaccination

Characterization and vaccination of the cytosolic Fasciola gigantica superoxide dismutase

10^th Euro Global Summit and Expo on Vaccines & Vaccination

June 16-18, 2016 Rome, Italy

Wipaphorn Jaikua

Mahidol University, Thailand

Posters & Accepted Abstracts: J Vaccines Vaccin

Abstract:

Superoxide dismutase (SOD) of Fasciola spp. belongs to a family of metallo enzymes which are anti oxidant enzymes to defend against reactive oxygen species (ROS) from the hostâ??s immune cells. Thus, superoxide dismutase is thought to be the target vaccine candidate against Fasciola spp. parasite. In this study, the full length cDNA encoding SOD of Fasciola gigantica (FgSOD) was cloned from adult parasites by polymerase chain reaction (PCR). The sequence of FgSOD showed 468 bp of an open reading frame (ORF), 60 bp 5â?? UTR, 126 bp 3â?? UTR and the putative FgSOD peptide comprising of 154 amino acid with a molecular weight of 17.5 kDa and PI 5.45. The recombinant FgSOD was expressed in Escherichia coli BL21 (DE3), purified by Ni-NTA chromatography under native condition. A vaccination was performed in imprinting control region (ICR) mice by subcutaneous injection with 50 μg of rFgSOD combined with Freundâ??s adjuvant. At 2 weeks after the second boost, mice were infected with 15 metacercariae by oral route. IgG1and IgG2a in the immune sera was determined to indicate Th2 and Th1 immune response. It was found that the parasite burden was reduced by 45% and both IgG1 and IgG2a levels showed correlation with the numbers of worm recoveries.

Biography :

Wipaphorn Jaikua is currently pursuing PhD in Pathobiology at the Faculty of Science, Mahidol University, Thailand.

Email: wiangni@hotmail.com