Journal of Bioequivalence & Bioavailability

Biotechnological exploitation of bacteria for PHB synthesis: Pharmaceutical applications

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

Khayyam N. Shaikh

Posters: J Bioequiv Availab

Abstract:

T here has been a considerable interest in development of biodegradable polymers such aspoly beta hydroxy butyrate(PHB) from bacterial origin which could help in solving probable problems due to use of synthetic polymers. Many synthetic polymers are being used now-a-days in drug delivery systems. But synthetic polymers have certain disadvantages such as their non-biodegradability and so probability of bioaccumulation. Such accumulations for long time in body are not good. This explains a need of easily biodegradable polymer. Bacteria can synthesize a wide range of biopolymers which are biodegradable, biocompatible andhave material properties suitable for medicalapplications. Bacterial polymers such as PHB, if modified to make functionally more effective can be better for use in pharmaceutical field. These studies started with screening of better producer of PHB from soil. This paper represents a work on screening of bacterial isolates capable of producing PHB, and production of PHB using laboratory scale fermentation procedures. Using nutrient agar we could screen four bacteria from soil, capable of producing PHB, using Sudan black-B staining. We also used a pure bacterial culture of Alcaligeneslatus obtained from MTCC Chandigarh as a producer of PHB. Out of these five bacterial isolates, Alcaligeneslatus found producing PHB in relatively more amounts yielding about 25%. We used Alcaligeneslatus for production of PHB in large quantity