Journal of Bacteriology & Parasitology

Biosynthesis of silver nanoparticles using Pseudomonas fulva, as a novel silver nanoparticles producing isolate

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Sajjad Yazdansetad

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Silver nanoparticles (Ag NPs) are the most interesting nanoparticles due to their wide variety of applications in various fields
especially biomedical. In contrast, a promising usage of Ag NPs as antimicrobial agent is well known and has already found
applications in antimicrobial paint coatings, textiles, water treatment, wound healing, medical devices, and HIV prevention
as well as treatment. In this study, we have reported the successful synthesis of silver nanoparticles by Pseudomonas fulva, as a
novel silver nanoparticles producing isolate. Pseudomonas fulva, a silver nanoparticles producing organism was isolated from
vinegar. The isolate was confirmed by standard biochemical tests and 16S rDNA typing method. The ability of the organism
was evaluated by adding 1 M solution of silver nitrate (AgNo3) to the microorganism culture medium. Production of the silver
nanoparticles was investigated by X-ray diffraction (XRD), spectrophotometry, transmission electron microscopy (TEM), and
fourier transform infrared spectroscopy (FTIR) analysis. The biosynthesis of silver nanoparticles was observed with the color
change of the microorganism culture medium from yellow to dark brown. The sterile culture medium (without microorganism)
supplemented with AgNo3 was used as a negative control. XRD indicated the silver nanoparticles diffraction peaks which were
ranged from 2θ=30° to 2θ=80°. A peak at 430 nm for silver nanoparticles was seen by spectrophotometer. TEM micrograph
revealed the fairly uniform size and spheral shapes of silver nenoparticles in the range of 50 to 100 nm. FTIR absorption spectra
with range of 500 to 3500 cm-1 were also observed for silver nanoparticles. Biosynthesis of nanoparticles by microorganism is a
green and eco-friendly technology. In this study, we introduced the novel silver nanoparticles producing isolate (Pseudomonas
fulva), for the first time. Our study indicated that the isolate has robust potential in production of silver nanoparticles. Further
study is required to explore the silver nanoparticles production of P. fulva.

Biography :

Sajjad Yazdansetad is PhD Candidate in Microbiology at Department of Microbiology, Science and Research Branch Univesity, Tehran, Iran. His main research
fields are molecular microbiology and biotechnology. He has presented over 10 papers in conferences. Now, he studies silver nanoparticles as a novel approach
in treatment of bacterial infections and biomedical applications.