Journal of Bacteriology & Parasitology

Biofertilizer based in endophytic bacteria for selenium biofortification and Gaeumannomyces graminis biocontrol

9^th Biotechnology Congress

August 31-September 02, 2015 Orlando,Florida, USA

Paola Duran

Universidad de La Frontera, Chile

Posters-Accepted Abstracts: J Biotechnol Biomater

Abstract:

In the last few years we showed that some microorganisms as bacteria and arbuscular mycorrhizal fungi can be used as biotechnological tools to enhance the Se content in plants. Here, endophytic bacteria (Acinetobacter sp. E6.2 and Bacillus sp. E5) were isolated from selenium-supplemented wheat plants and were characterized in terms of Se accumulation and capacity of biocontrol of Gaeumannomyces graminis vartritici (Ggt), the main soil borne pathogen of southern Chile. Both strains were highly tolerant to elevated selenium concentration (ranged from 60 to 120 mM respectively) and showed potential plant-growth-promoting capabilities (auxin and siderophore production, phytate mineralization and tri-calcium phosphate solubilization). In terms of Se species we found that mainly Acinetobacter sp. E6.2 produced elevated amounts of SeMet and SeMeSeCys (10 and 3.77 mg kg-1 respectively), however highly stable NanoSe (Z potential around -40 mV) was the main Se form found in both inoculums. The size of NanoSe from Acinetobacter sp. was major than Bacillus sp. along the time (i.e., 213±3.4 nm and 169±0.92 nm respectively at 24 hours). In addition, Se supplementation in bacteria no produced an oxidative stress measured by antioxidant activity (SOD and CAT) and neither affected the tolerance to water deficit due to similar proline and IAA production. Respect to pathogen biocontrol Acinetobacter sp. was able to inhibit 100% pathogen development, whereas 30% was inhibited by Bacillus strains. Our results validate the potential use of these endophytic bacteria inoculums for Se biofortification and Ggt biocontrol.

Biography :

Paola Durán is a Post-doctoral Researcher in la Frontera University She has completed her PhD from the University of Barcelona obtaining “Cum Laude” distinction. She is a Professor of Microbiology in Plant-Soil System. She has published 9 papers in the last 5 years in ISI journal and has been serving as an Editorial Board Member of repute.

Email: paola.duran@ufrontera.cl