Journal of Vaccines & Vaccination

Biodegradable protein nanoparticle from Bombyx mori, as a vehicle for delivery of vaccines

7^th Middle East - Global Summit and Expo on Vaccines & Vaccination

September 28-29, 2015 Dubai, UAE

M Krishnan, N Pauline Renitta and N Jothi

Bharathidasan University, India

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

Nanotechnology, although not a new concept, has gained significant impetus in recent years. Due to the recent advances in material science and nano-engineering, the nanoparticles have become very attractive for their applications in the fields of biology and medicine. For the past few decades, there has been a considerable research interest in the area of drug, vaccine/ gene delivery using particulate delivery systems as carriers for small and large molecules. The biodegradable nanoparticles are gaining momentum and offer a suitable means to deliver small molecular weight drugs as well as macro-molecules such as proteins, peptides or genes in the body using various routes of administration. However, there are several issues related to the delivery of polynucleotides which limit their application. These issues include efficient delivery of the polynucleotide to the target cell population, its localization to the nucleus of these cells and ensuring that the integrity of the polynucleotides is maintained during delivery to the target site. Nano particles loaded with plasmid DNA could also serve as an efficient sustained release of gene delivery system due to their rapid escape from the degradative endo-lysosomal compartment to the cytoplasmic compartment. In our present study, we prepared the biodegradable protein nanoparticle from pupal waste of Silkworm, Bombyx mori by polymerization method. It was further characterized and confirmed through UV, FTIR, Zetapotential and TEM analysis. Interestingly, the pupal waste nanoparticles do not have any toxic effect on silkworm when compared to the plant based silver nanoparticle. Further, we are working towards the optimization of delivery system for polynucleotide coated biodegradable nanoparticle.

Biography :

M Krishnan is a Lecturer in Bharathidasan University. He got fund from World Bank to improve the seed and silk production in Bombyx mori. The Central Silk Board, a pioneer Government agency to look into the improvement of sericulture industry in India, has taken up the result obtained in his laboratory and recommended the same to sericulture farmers and also for patenting (Indian patent PATA/4.38/20029/dt 18.03.2005). His genuine contribution is towards the improvement of the Sericulture society, an agro-based small scale industry in India. It also pays the way to improve the employment for the rural people. Following this, his research work focused on identifying the proteins such as storage and vitellogenin protein which are heavily regulated by the nutrition and the same were exploited for the pest management. He has established a Molecular Biology Laboratory at Bharathidasan University. He has developed his experimental knowledge in protein purification and mass spectrometric analysis (MALDI-TOF, MALDI-MS/MS and LC-MS) in the University of Munster, Germany through INSA award. At present, he is concentrating towards the implementation of Molecular Biology along with Nanotechnology, as a novel tool to find resolution for human welfare.

Email: profmkrish@gmail.com