Health Care : Current Reviews

Au/ZnO nanocomposites prepared via laser ablation for enhancement of antibacterial activity and cytotoxic properties against AMG13-SKG-4 line

International Congress on Global Healthcare

August 24-25, 2023 | WEBINAR

Taha M. Rashid

Phi nanoscience center, Iraq

Scientific Tracks Abstracts: Health Care Curr Rev

Abstract:

Statement of the problem: This study presents a comprehensive look into the potential therapeutic, antibacterial, and anticancer properties of a nanocomposite (NC) of gold (Au) and zinc oxide (ZnO). We analyzed the adherence between Au nanoparticles (NPs) and ZnO NPs. X-ray diffraction analysis showed high crystallinity and small crystallite sizes of Au NPs and ZnO NPs, while transmission electron microscopy showed spherical NPs. Furthermore, histogram analysis showed that the average particle size of Au NPs is 27 nm, while that of ZnO NPs is 35 nm. The adherence of ZnO NPs on the surface of Au NPs increased their combined particle size to 51 nm and revealed a high population core-shell structure. The activity of Au/ZnO NCs against Escherichia coli was more potent when compared to that of elemental Au and ZnO NPs alone. The cytotoxic effects of Au NPs, ZnO NPs, and Au/ZnO NCs against human breast cancer cells (AMG13) and human esophageal adenocarcinoma cancer cells (SK-GT4) were investigated in this study. Methodology & Theoretical orientation: prepared Au NPs and ZnO NPs by green synthesis (laser ablation and study their activity against Anticancer and antimicrobial, An Au plate was immersed in 3 mL of deionized water (DW) in a glass vial and ablated with 1064 nm pulses from an Nd:YAG laser, frequency at 1 Hz with a pulse duration of 9 ns. The ablation was performed for 200 pulses with a laser energy of 800 mJ directed at the target, Then, 3 mL of DW was added to a glass beaker with a Zn pellet at the bottom and irradiated separately under the same conditions used above, the Au NP solution went from pale pink to deep pink, while the ZnO NPs solution went from pale gray to pale yellow. To prepare Au/ZnO NCs, different volumetric ratios for Au NPs with ZnO NPs (1:3, 1:1, and 3:1 v/v) were used. After mixing two colloidal solutions, the second harmonic laser pulses of 800 mJ for 100 pulses were directed at the surface of the solution. The wavelength of the laser used was 532 nm. Findings: AMG13 is more sensitive than SK-GT4 to the activity of Au/ZnO NCs. The cytotoxicity of Au/ZnO NCs against AMG13 was 89%, while that against SK-GT4 was 85%. Less cytotoxic effects were recorded against normal cells (MCF7) when compared with those of cancer cells. Based on these findings, the synthesized Au/ZnO NCs could be used as a promising strategy for biomedical applications. Conclusion: Successful preparation of Au NPs, ZnO NPs, and Au/ZnO NCs was produced, which tested for their antibacterial potentials against E. coli and their cytotoxic properties against the breast cancer AMG13 cell line and the esophageal cancer SK-GT4 cell line. In the prepared Au/ZnO NCs, the Au NPs were spherical in shape with a size of around 27 nm, and the ZnO was polycrystalline in nature with a wurtzite structure. Compared with the ZnO, the Au/ZnO NCs exhibited increased light absorption and a widened photo-response, indicating that the incorporation of Au NPs with ZnO NPs increased the capacity of UV and visible light absorption, and hence enhanced the photogeneration rate of electrons and holes by light excitation in a wide spectral region. Au and ZnO NPs showed promising antibacterial activity and were more effective when combined as an NC in varying volumes. The results demonstrated that breast cancer cells were shown to be more sensitive than esophageal cancer cells. The proliferation rate of normal breast cells demonstrated a less cytotoxic effect when compared with those observed in cancerous cells. Based on current outcomes, the prepared Au/ZnO NCs could represent a promising strategy for biomedical applications.

Biography :

Taha M. Rashid, Ph.D, UOT, Applied Physics department - Currently works at the Department of haematology and therapeutic, Almustansiriysh university. CEO of phi nanoscience center, Iraq-Baghdad. Our team researchers working on study the activity of nanoparticles against the cancer cell lines.