Journal of Bacteriology & Parasitology

Antimicrobial resistance genes in Salmonella isolates from poultry drinking water

4^th International Congress on Bacteriology and Infectious Diseases

May 16-18, 2016 San Antonio, USA

Nadia Mukhtar

Western University, Canada

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Salmonella species are among the most common causes of human bacterial gastroenteritis worldwide and food animals specially poultry are important reservoirs of this bacteria. In recent years, due to increase in the occurrence of antimicrobial resistance spp. of Salmonella, fatality rate for Salmonellosis is increasing significantly. In the context of exploring the emergence of antimicrobial resistant Salmonella species, present study was planned. Salmonella isolation was performed by collecting samples from poultry rearing and slaughtering areas with recovery rate about 29.3%. Bacterial colonies of red color with black center were appeared on the XLD agar plates and then confirmed by biochemical tests. Then multi-drug resistance of the isolates was examined by disk diffusion method. Resistant samples were further analyzed for the detection of various tet genes (tetA, tetB, tetC, tetD, tetG). PCR confirmed the presence of tetA in all the Salmonella positive samples while tetB was present in combination with tetA gene only in 16 samples. No amplification of tetC, tetD and tetG was examined. Our results illustrate that commonly used antibiotics, especially tetracycline is showing decline in efficacy due to increasing antimicrobial resistance in locally isolated Salmonella from poultry samples. These findings indicate that local population of Salmonella contains the tetA alone or in combination with tetB gene and are likely played an important role in transmission of antimicrobial resistance determinants among Salmonella strains.

Biography :

Nadia Mukhtar is currently a PhD student in Department of Microbiology at University of Veterinary and Animal Sciences of Lahore. She is currently working as an Instructor Microbiology at Virtual University of Pakistan. She has published more than 10 papers in reputed journals. She has won a Scientific Exchange Award as the part of AAAS BMENA Scientific Exchange Program, supported by a grant from the U.S. Department of State, 2013. She got training on Metagenomic investigations of respiratory infections of Sheep at Harvil’s Lab, Penn State University, USA, 2014.

Email: nadia.mukhtar@vu.edu.pk