Whika Febria Dewatisari
Scientific Tracks Abstracts: J Bacteriol Parasitol
Sansevieria trifasciata used as an ornamental plant. It also used as a traditional medicine for influenza, cough and inflammation
of the respiratory tract. The roots and leaves of S. trifasciata contains many secondary metabolites such as saponins that
efficacious as a cough remedy to treat sprains, injuries hit, venomous snake bites, ulcers, cough, inflammation of the respiratory
tract and hair growth. Microbes are used to seeing the antibacterial saponins activities of S. trifasciata are Escherichia coli and
Staphylococcus aureus. The main reason for used these microbes because E. coli is a bacterium that causes diarrhea and S.
aureus is one of the bacteria that cause cough in humans. Plants were used S. trifasciata var. Golden Hahnii. Based on research
by Dewatisari (2008), states that the variety had the highest saponin content of S. trifasciata is Golden Hahnii among other
varieties and the parts that had the highest saponin were in its roots. This article aims to explain quantitatively the effectiveness
of saponin compounds from the roots of S. trifasciata var. Golden Hahnii as anti-microbials in inhibiting the growth of bacteria
S. aureus and E. coli. Methods of data collection were sample preparation, a preliminary test, the extraction of saponins,
antibacterial activity test and isolate compound separation by TLC (Thin layer Chromatography). This study includes the
extraction of the active compounds in the roots of S. trifasciata by maceration with methanol 90%. Separation of the active
compound was conducted by TLC. Eluent used was chloroform; methanol; water with various concentration (13: 4: 1), (65; 50:
10), (20: 60: 4), 20: 60: 10). Antibacterial test conducted by the disc diffusion method against S. aureus and E. coli. Identification
of test compounds triterpenoid saponins using foam and Liebermann-Burchard color test (LB). The results showed that the
extract of the roots has potential as anti-bacterial. The extract was able to inhibit the growth of E. coli and S. aureus. At the
optimum concentration of 200 ppm produced inhibition zone was 26.5 mm to 20.2 mm for S. aureus and E. coli. Best eluent to
separate the triterpenoid saponins in root extracts of S. tifasciata was chloroform; methanol; water at a concentration (20: 60:
4) with 3 separate visible stains are: 0.135; 0.85; 0.815. Mechanism root extract as an anti-bacterial S. trifasciata was synergistic.
It was seen from the inhibition zone, for E. coli isolates I=5.52 mm and isolates II=2.50 mm, for S. aureus isolates I=1.52 mm
and isolates II=0.58 mm while the isolates III is not effective as an anti-bacterial.
Whika Febria Dewatisari is an Academic Staff at Department of Mathematic and Natural Science in Open University (Universitas Terbuka) Bandar Lampung-a
public university in Indonesia. She is a Biology Lecturer and started working at 23 years old. She teaches courses in Plant Embriology, Microbiology and Ecology.