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Journal Flyer
Journal of Microbial & Biochemical Technology
Antifungal metabolites from sponge associated marine Streptomyces sp. strain (ERIMA-01) from south east coast of India
2nd International Conference on Clinical Microbiology & Microbial Genomics
September 16-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Naif Abdullah Al-Dhabi

Posters: J Microb Biochem Technol


Streptomyces sp. strain ERIMA-01 was isolated from the marine sponge collected from Tsunami affected area of south east coast of India. The strain was grown in ISP 2 medium to study the morphology and biochemical characteristics. The strain was subjected to 16S rRNA and identified as Steptomyces sp. The Streptomyces sp. Inhibited the growth of all the fungi when tested using the streak method. The strain was grown in different media. The fermented broth was collected and tested against fungi. The broth showed good antifungal activity. Antifungal metabolite production of ERIMA-01 was evaluated using five different fermentation media. Most active broth (MNGA) was extracted with ethyl acetate. The ethyl acetate extract inhibited the growth of all the tested fungi ( Trichophyton mentagrophytes at 62.5 μg/ml, Trichophyton rubrum at 31.25 μg/ml, Trichophyton simii at 125 μg/ml, Scopulariopsis sp at 125 μg/ml, Aspergillus flavus at 250 μg/ml, Aspergillus niger at 62.5 μg/ml, Botrytis cinerea at 125 μg/ml, Candida albicans at 15.2 μg/ml, Candida krusae at 31.25 μg/ml, Candida tropicalis at 125 μg/ml and Candida parapsilosis at 125 μg/ml. Most active ethyl acetate extract was subjected to GC-MS analysis which showed 38 components. The major compound was 1- tetradecanol (11.59%).