Clinical & Medical Biochemistry

Amelioration of renal and hepatic function by Nepalese plant extract in gentamicin induced renal toxicity in rat model

14^th International Conference on Biosimilars and Pharma Innovations

November 09, 2022 | Webinar

Sindhu K C

Pokhara University, Nepal

Posters & Accepted Abstracts: Clin Med Bio Chem

Abstract:

Statement of the Problem: Gentamicin is a widely used beneficial antimicrobial drug. Despite of its beneficial role in health system, certain concentration and its long term administration leads to renal injury through the mechanism of oxidative stress. Purpose: The aim of the present study was to assess the qualitative and quantitative evaluation of phytochemical constituents present in selected plant species extract along with determination of its antioxidant, antiinflammatory potency through in-vitro method, followed by selecting the species extract on the basis of their in-vitro and phytochemical result for further study in in-vivo experimental acute toxicity, along with their effect on nephrotoxicity induced by gentamicin and also its remote organ injury in the liver. Finding: Qualitative evaluation of phytoconstituents indicate the presence of phenols, flavonoids, tannins, glycosides, carbohydrate in selected Nepalese Plant species where quantification of total flavonoids in AB extract and total phenol content were found to be high among selected species of plant extract. In-vitro oxidative stress was assessed through DPPH scavenging activity. Among selected species highest DPPH scavenging potency was obtained from AB extract (IC50 values 4.53μg/ml) and all three species showed highest % of HRBC stabilization potency at low dose of concentration. Among all of the species AB extract was selected that showed median lethal dose (LD50) to be above 5000 mg/kg body weight. Which is able to restore the increased serum levels of Creatinine, urea, AST, ALP by (P< 0.001) when compared with toxic group, with restoration of the increased weight of kidney by (P< 0.01) along with improvement in tissue injuries of kidney and liver. Conclusion: Thus the AB extract has potential role in ameliorating the renal function and hepatic function in gentamicin induced nephrotoxicity.