Journal of Bioequivalence & Bioavailability

Ability of 3 extraction methods (BCR, Tessier and Protease K) to estimate the bioavailable metals in sediments from Huelva Estuary, southwestern Spain

7^th World Congress on Bioavailability & Bioequivalence: BA/BE Studies Summit

August 29-31, 2016 Atlanta, USA

Daniel Rosado

Universidad de Sevilla, Spain

Posters & Accepted Abstracts: J Bioequiv Availab

Abstract:

We estimated the bioavailable fraction of metals (Zn, Cu, Cd, Mn, Pb, Ni, Fe, and Cr) in sediments of the Huelva estuary and its littoral of influence by carrying out the most popular methods of sequential extraction (BCR and Tessier) and a biomimetic approach (extraction with protease K). We compared the results obtained with enrichment factors found in Arenicola marina. The linear correlation coefficients (R2) obtained between the fraction mobilized by the first step of the BCR sequential extraction; by the sum of the first and second steps of the Tessier sequential extraction; and by protease K. The enrichment factors in Arenicola marina are at their highest for protease K extraction (0.709), followed by BCR first step (0.507) and the sum of the first and second steps of Tessier (0.465). This observation suggests that protease K represents the bioavailable fraction more reliably than traditional methods (BCR and Tessier), which have a similar ability.

Biography :

Email: danrosalc@alum.us.e