Wen-Qi Liu, Fei Guan, Hong Xu, Bing-Tao Su, Jia-Hui Lei and Yong-Long Li
Huazhong University of Science and Technology, China
Posters-Accepted Abstracts: J Bacteriol Parasitol
Excretory-secretory products (ESPs) are the majority of worm components which can be directly recognized by host immune system. In our current study, a proteomic approach combined by MS with Two-Dimensional Gel Electrophoresis (2-DE) was used to identify ESPs of S.japonicum. By 2-DE, we identified 166 protein spots from adult ESPs and 138 protein spots from egg ESPs. Of these spots, twelve protein spots were identical in both eggs and adult worms, 24 spots were stage-specifically expressed in adult worms and 16 spots were egg-specifically. In analysis of MALDI-TOF/TOF, the abundant proteins of adult worms revealed a confident constituent of enzymes, structural proteins, and response elements. Of these, four molecules could be recognized by infective sera of two weeks, and two were reactive with sera of six weeks post infection. An enolase screened from egg ESPs was found to strongly react with early-infected sera, and another putative CAP-Gly-domain protein from egg ESPs was also weakly recognized by sera of early infection. Transposase, Abhydrolase Domain-Containing Protein 8 (ABHD- 8), and other five hypothetic proteins were not be detected by immune-blotting. In brief, and also notably, we identified total 166 and 138 proteins from ESPs of adult worms and eggs, respectively, which mainly involved in redox balance, protein folding, development and signaling, scavenging, metabolic pathways, and immune response modulation. Several uncharacterized S. japonicum proteins have also been reported in present study. Moreover, five antigens, including confident proteins and putative proteins, have been screened to be potential in early diagnosis for schistosomiasis.