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Research Article - (2013) Volume 4, Issue 8

Simultaneous Determination of NSAID and Antimicrobial Preservatives Using Validated RP-HPLC Method: An Application in Pharmaceutical and Clinical Laboratories

Najmul Hasan1*, Mathurot Chaiharn2, Shabana Naz Shah3, Hira Khalid4 and Abdul Jabbar5
1Department of Microbiology, Faculty of Science, University of Karachi, Karachi-75270, Pakistan
2Division of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand
3Department of Pharmaceutical Chemistry, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi-75270, Pakistan
4Department of Chemistry, Government College University Faisalabad, Lahore, 54000, Pakistan
5Provincial TB Control reference laboratory, National TB control Programme Peshawar, Pakistan
*Corresponding Author: Najmul Hasan, Department of Microbiology, Faculty of Science, University of Karachi, Karachi-75270, Pakistan, Tel: 0092-333-366-9331 Email:

Abstract

A stability indicating, accurate, specific, precise and simple high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Dexibuprofen along with its preservatives i.e. Sodium Benzoate, Methylparaben, and Propylparaben in pharmaceutical dosage forms of oral solution and in serum. Acetonitrile: Acetate Buffer: Acetic Acid (0.1 M) (50:50:0.3 v/v/v) (pH 5.5) was the mobile phase at flow rate 1.0 mL min-1 using a Hibar® μBondapak® C18 column monitored at wavelength of 230 nm. The calibration curve was linear with a correlation coefficient of more than 0.9995 for the drugs. The averages of the absolute and relative recoveries were found to be 100.07%, 99.82%, 99.91% and 99.97% for Dexibuprofen, Sodium Benzoate, Methylparaben and Propylparaben respectively with 5 to 25 ng mL-1 limit of quantification and 1.5 to 7 ng mL-1 limit of detection. The drugs were subjected to stress conditions of hydrolysis (acid, base, oxidation, and thermal degradation). Maximum degradation was observed in base and 35% H2O2 while found almost stable in the other stress conditions. The studies of forced degradation prove the stability indicating power of the method. The developed method was validated in accordance to ICH guidelines. The proposed High-performance liquid chromatographic method was successfully applied to quantify the amount of Dexibuprofen and three most common microbial preservatives in bulk, dosage form and physiological fluid.

Keywords: Dexibuprofen; Oral solution; Paraben; Benzoate; RPHPLC; Serum

Abbreviations

MP: Methylparaben; PP: Propylparaben; SBZ: Sodium benzoate; DBPN: Dexibuprofen; HPLC: High Performance Liquid Chromatography; ICH: International Conference on Harmonisation; NSAID: Non-Steroidal Anti-Inflammatory Drug

Introduction

Dexibuprofen (DBPN, Figure 1) is the pharmacologically effective (S+ Enantiomer of Ibuprofen) and chemically is 2-[4-(2-methylpropyl) phenyl] propanoic acid [1]. In contrast to Ibuprofen the administration of dexibuprofen may offer the advantage of delivering the well-known antipyretic, analgesic and anti-inflammatory properties of ibuprofen in a more efficient way, with a better safety profile than the racemic ibuprofen formulations [2]. It is a widely used as nonsteroidal antiinflammatory and analgesic agent for many years [3,4] and for the symptomatic treatment of osteoarthritis, primary dysmenorrhoea, muscular skeletal pain or dental pain and reduces gastric damage and improves analgesic and anti-inflammatory effect than racemic ibuprofen [5]. Dexibuprofen is rapidly absorbed, and peak serum concentration is achieved within 1.5-2 hours of oral administration [6]. It has been established there is a significant correlation between serum Dexibuprofen levels and the resultant degree of pain relief, particularly 1 hour after administration [7].

pharmaceutica-analytica-acta-propylparaben

Figure 1: Chemical structures of Dexibuprofen (DBPN) Methylparaben (MP) and Propylparaben (PP).

Methylparaben (C8H8O3) (MP Figure 1) Propylparaben (C10H12O3) (PP, Figure 1) and Sodium benzoate NaC6H5CO2 are the well-known preservatives that are used primarily for their bactericidal and fungicidal properties. Parabens that include MP and PP, are a group of the alkyl esters of p-hydroxybenzoic acid, have a low toxicity profile and a long history of use. It readily absorbed from the gastrointestinal tract or through the skin [8]. Usage of MP is toxic at higher concentrations, has an estrogenic effect [9]. The estrogenic activity of parabens increases with the length of the alkyl group and is believed that PP is estrogenic to a certain degree as well [10]. Sodium benzoate (SBZ) as a cheap preservative inhibits bacteria, mold & yeast under acidic condition. The Food and Drug Administration limits sodium benzoate to concentrations of 0.1% by weight. Therefore for the reassessment of the safety and significance of these preservatives a new, simple method was needed to develop along with effective Dexibuprofen.

Literature survey reveals some analytical methods for Dexibuprofen including High-Performance Liquid Chromatographic (HPLC) determination of Dexibuprofen in St. John’s Wort [11-13], in human serum and other physiologic fluids by HPLC/HPLC-mass spectrometry [14-19], by radian compression technique [20].

Some methods of analysis of MP, PP either alone or in some other formulations are available including High-Performance Liquid Chromatographic i.e. [21-32], gradient HPLC [33], solid phase extraction HPLC [34], HPLC-MS [35].

However to the best of our extensive research survey revealed that till now no stability indicating HPLC method is available which can determine the four ingredients concomitantly, therefore an endeavor was made to develop a simple, sensitive and validated stability indicating RP-HPLC method using UV detection to determine Dexibuprofen along with its essential microbial preservatives in pharmaceutical dosage forms and human serum. The present work deals with method development, method validation and forced degradation study of the ingredients. The applicability of the method was confirmed for analysis in pharmaceutical products and for pharmacokinetic studies. The results of analysis were validated in accordance with ICH guidelines [36].

Materials and Methods

The present method was designed to be easy to use, sensitive, rapid and simple sample preparation for the active and preservatives ingredients. Separation and quantification in pharmaceutical drug formulations and blood were achieved with an isocratic elution.

Reagents and chemicals

Dexibuprofen, Sodium Benzoate, Methylparaben, Propylparaben were kind gifts from National Pharmaceuticals, whereas sulphuric acid, sodium heptane sulphonate, Acetonitrile (HPLC grade) were purchased from Merck (Germany). The pharmaceutical dosage forms containing the respective ingredients were obtained from commercial source (DESOMIN®, DEXACT®, DEXIB®, and ZIBUFEN®) labeled to contain 20 mg mL-1, 0.5 mg mL-1, 0.2 mg mL-1 and 0.1 mg mL-1 of DBPN, SBZ, MP & PP respectively. Distilled water was obtained by passage through RO plant (Waterman, Pakistan) and was further filtered through a 0.45 μm membrane filter (Millipore, Bedford, MA, USA).

Instruments

For chromatography a SIL 10A auto injector HPLC system comprising of SCL 10A system controller, SPD 20A prominence UV/VIS detector, and Shimadzu LC 20 AT pump with LC Solutions software was used. Separation was performed on a Hibar® μBondapak® ODS C18 HPLC column, (4.6×250 mm; 5 μm bead size) maintained at 25°C. A UV-visible Shimadzu 16500 PC spectrophotometer with UV Probe software, ultrasonic cleaner (Elmasoni E 60 H), Jenway 3240 pH meter and Sartorious TE2145 analytical balance were used in the research work. Throughout the work only amber glass flasks were used to avoid light effect on the solutions of DBPN, SBZ, MP & PP standards and samples.

Chromatographic conditions

The HPLC analysis was carried out at ambient temperature. The compound was chromatographed isocratically with a mobile phase consisting of Acetonitrile (HPLC grade): Acetate Buffer 0.1M (Disodium hydrogen phosphate): Acetic Acid 0.1M (50:50:0.3, v/v/v) with the apparent pH adjusted if required to 5.5 ± 0.1 using Acetic Acid 0.1 M. The mobile phase was filtered by passing through a 0.45 μm membrane filter (Millipore, Bedford, MA, USA). The flow rate was 1.0 mL min-1, and the injected volume was 20 μL. The effluent was monitored spectrophotometrically at wavelength of 230nm. Mobile Phase was used as dilution solvent.

Analytical procedure

Standard preparation: SBZ, MP& PP reference standard were accurately weighed as 50 mg, 20 mg and 10 mg in 100 ml flask and dissolved in diluent to have a stock solution-A of 500 μg mL-1, 200 μg ml-1 and 100 μg mL-1 respectively, while in another 100 ml volumetric flask, accurately 100 mg of DBPN was weighed and dissolve in 70ml of dilution solvent sonicated for 5 minutes and then added by 5 ml of the stock solution-A and filled up to the mark with dilution solvent to get stock solution-B containing 1000 μg mL-1 of DBPN, 25 μg mL-1 of SBZ, 10 μg mL-1 of MP and 5 μg mL-1 of PP. The solution-B was used in further analytical steps.

Sample preparation

Assay of suspension containing the drug: For making a sample from suspension, 10 bottles were opened and content was mixed to get an evenly homogenized stock sample. The sample density was determined and then sample weight accurately equivalent to 5 ml was taken in 200 ml volumetric flask and 100 mL of diluent was added. The sample was sonicated for 5 minutes and then added diluent up to the mark, placed on stirrer for 10 minutes. The sample was then filtered through 0.45 μm filter paper and injected into the HPLC system.

Sample preparation for serum: Blood samples were collected from healthy volunteers in evacuated glass tube through an indwelling cannula placed on forearm vein by a trained clinical laboratory technician. The volunteers were not involved in any medication, smoking, and strenuous activity. The blood was shaken and centrifuged at 10,000 rpm for 10 min to separate out serum. Acetonitrile was added to serum (90:10, v/v) and centrifuged at 10,000 rpm for 10 min to deprotonated it. The serum thus obtained was filtered and used for the analysis and were stored at 20°C. For making working sample 10 ml of stock solution-B was taken in 20ml flask, followed by 5 ml of serum. The sample thus obtained was stir for 10 minutes and then added diluent up to the mark. Triplicate solutions were made for each working solution for the analysis in serum, was filtered through 0.45 μm filter paper and injected into the HPLC system.

Assay of suspension for degradation studies of drugs: For this purpose 25 ml of stock solution-B was diluted in four individual 50 mL volumetric flasks and 15 mL of degrading agent were added to each flask individually, with exception of one to which only diluent was added; these included 0.1M HCl, 0.1M NaOH, 35% H2O2 and then to each flask diluent was added up to the mark. All the four samples were placed in water bath at 60°C for one hour. The samples were then filtered through 0.45 μm filter paper and injected into the HPLC system.

Stability studies

For stability studies the commercially available suspension samples were placed at accelerated conditions of temperature that is at 40°C with 75% relative humidity and at ambient conditions of 30°C temperature with 65% relative humidity in environmental chamber for six months. The stability protocol showed elsewhere was followed for six months and assays were made as described in method development.

Intentional degradation was attempted to stress conditions exposing the drugs with acid (0.1N HCl), alkali (0.1N NaOH), hydrogen peroxide (35%) and heat (60°C) to evaluate the ability of the proposed method to separate drugs from its degradation products. For all conditions the heat temperature was kept constant at 60°C for a period of one hour.

Method validation

The method validation was performed in following ICH guideline [36] according to which the assay validation was performed via various procedures including specificity, linearity, range, accuracy, intra-day and inter-day precision etc.

To study the linearity of standard solutions, twenty dilutions were prepared from stock solution-B to give standard solutions in range of 10% to 200% of drugs content. The standard calibration curve was generated using regression analysis. For specificity commonly used excipient in suspension preparation were spiked in a pre-weighed quantity of drugs and then peak areas were measured and calculations done to determine the quantity of the drugs recovered.

The precision was by analyzing corresponding standard daily for a period of three days i.e. Inter-day precision, and three times a day with an interval of 8 hours (Intra-day precision) against freshly prepared standard solutions. For determining accuracy the reference standards were accurately weighed and added to the suspension sample, to get three different concentration levels i.e. 80% -100% -120% of the ingredients. At each level, samples were prepared in triplicate and the recovery percentage was determined.

Limit of detection and quantification (LOD & LOQ) for the method were established by sequential diluting the standard solutions at decreasing concentrations, in the range of 100 ng mL-1 to 1 ng mL-1 and injected onto the chromatographic system. The limit of detection was defined as the concentration for which a signal-to-noise ratio of 3 was obtained and, for quantification limit; a signal-to-noise ratio of 10 was considered.

The robustness was studied by analyzing the same samples of suspension by deliberate variation in the method parameters, such as in the chromatographic conditions, like mobile phase, pH, flow rate, temperature etc. System suitability of the method was evaluated by analyzing the symmetry of the standard peaks, resolution and theoretical plates of the column.

Results and Discussion

The HPLC method development and its validation are the utmost requirements for any drug available in the market to have high quality products. A few methods are available for determination of the DBPN as described earlier, but many of them are used only for certain definite objectives and none can be generalized for its simultaneous determination with preservatives in form of pharmaceutical products and serum. Similarly none of them is as much sensitive as ours is; in terms of its Precision, accuracy, %recovery, limit of detection (LOD) and limit of quantification (LOQ). Moreover this method is sensitive enough to be used for pharmacokinetic studies as its LOD & LOQ are in nanogram range.

Method development & optimization

For developing an efficient method for analysis, parameters, such as detection wavelength, mobile phase composition, optimum pH and concentration of the standard solutions were comprehensively studied. All the ingredients were diluted in dilution solvent and then run through UV spectrophotometer in UV range of 190 nm-400 nm to get maximal wavelengths, where maximum absorbance was gained i.e. 266 nm, 220 nm, and 254 nm for DBPN, SBZ and MP & PP respectively. However considering the difference of concentration of the ingredients and intensity of their absorbance a single wavelength method was adopted, that is 230 nm at which all the molecules gave a satisfactory absorbance. The chromatographic parameters were evaluated using a Hibar® μBondapak® C18 column. Mobile phase of an already developed method by our research team [37] was selected in terms of its components and their proportions and was modified. The mobile phase composed of Acetonitrile: Buffer of previously mentioned proportion promoted a short run time (20 min) as shown in (Figure 2), so this condition was adopted in subsequent analysis.

pharmaceutica-analytica-acta-chromatogram

Figure 2: Typical chromatogram of standard.

Validation studies

The linearity was determined in the range of 10%-200% for all ingredients. The assay was judged to be linear as the correlation coefficient was greater than 0.995 in all cases, calculated by the leastsquare method. A linear correlation was found between the peak areas and the concentrations, in the assayed range. The regression analysis data are presented in Table 1.

Parameter Inference
SBZ MP PP DBPN
Linearity range (µg /ml) 1.25-25 0.5-100 0.25-50 50-1000
Intercept(c) -5155.365 0 0 0
Standard Regression equation (y=mx+c) Slope(m) 3004.60 x -5155.365 1106.39 x + 0  1155.24 x + 0 2202.41 x + 0
Correlation coefficient (R2) 0.99986 0.99967 0.99952 0.99961
Limit of detection (LOD) (ng /ml) 3 7 5 1.5
Limit of quantification (LOQ) (ng /ml) 10 25 15 5

Table 1: Calibration curve data and validation parameters.

Chromatogram shown in Figure 3 proves specificity or selectivity of the assayed method, as the chromatograms in samples were found identical with standard chromatogram and no interference peak was observed. Peak purities higher than 98.0% were obtained in the chromatograms of sample solutions, demonstrating that other compounds did not co-elute with peaks of interest. The chromatogram obtained with the mixture of the suspension excipient proves that there is no interference from excipient and peak of interest fulfill all the requirements of symmetrical peak, and hence the specificity is confirmed.

pharmaceutica-analytica-acta-chromatogram

Figure 3: Typical chromatogram of physiological sample.

The precision of an analytical method is the degree of coherence among individual test results when the method is applied repeatedly to multiple sampling of homogeneous bulk. Intra-day precision of the method was evaluated at three different independent concentrations i.e. 80%, 100%, and 120% for the drugs (n=3) by determining their assays. Inter-day precision of the method was tested for 3 days at the same concentration levels. Solutions for calibration curves were prepared every day on freshly basis. Since the inter-day and intra-day precision obtained %RSD was less than 2% it assures that the proposed method is quite precise and reproducible as shown in Table 2.

Active Drugs Nominal Concentration (µg /ml) Day 1 Day 2 Day 3 Mean Inter-Day RSD%
DBPN 400 99.74% 100.38% 98.86% 99.66% 0.765
500 100.65% 100.96% 100.00% 100.53% 0.487
600 101.57% 98.95% 99.51% 100.01% 1.380
Mean 100.65% 100.09% 99.46% 100.07%  
Intra-Day RSD% 0.91 1.03 0.58 0.44
SBZ 10 100.78% 99.65% 99.74% 100.06% 0.628
12.5 99.74% 100.38% 98.86% 99.66% 0.765
15 101.21% 98.30% 99.71% 99.74% 1.459
Mean 100.58% 99.44% 99.44% 99.82%  
Intra-Day RSD% 0.75 1.06 0.50 0.21
MP 4 99.44% 100.83% 99.50% 99.92% 0.786
5 100.28% 99.92% 98.99% 99.73% 0.664
6 101.15% 100.70% 98.39% 100.08% 1.480
Mean 100.29% 100.48% 98.96% 99.91%  
Intra-Day RSD% 0.85 0.49 0.56 0.18
PP 2 99.65% 99.81% 100.98% 100.15% 0.725
2.5 98.56% 101.24% 100.62% 100.14% 1.401
3 99.98% 100.54% 98.40% 99.64% 1.114
Mean 99.40% 100.53% 100.00% 99.97%  
Intra-Day RSD% 0.75 0.71 1.40 0.29

Table 2: Inter-day and intra-day precision and recovery studies.

The accuracy was investigated by means of addition of reference standards to a mixture of the suspension excipients. Recovery studies of the drug were carried out for the accuracy parameter at three different concentration levels i.e. multiple level recovery studies. A known amount of API’s standards were added into pre-analyzed sample and subjected to the proposed HPLC method. The mean recovery (n=9) was 99.46%-100.65% for DBPN, 99.44%-100.58% (RSD=0.77%) for SBZ, 98.96%-100.48% (RSD=0.95%) for MP and for PP 99.40% -100.53% (RSD=0.95%), demonstrating the accuracy of the method. Percentage recoveries for marketed products were found to be within the limits (Table 3).

SAMPLES Content (%) ± S.D.
DBPN SBZ MP PP
Desomin® 99.75% ±  0.19 99.87% ±  0.69 97.77% ±  0.73 98.91% ±  0.34
Dexact® 98.91 % ±  0.81 100.62% ±  0.97 99.78% ±  0.11 99.85% ±  0.31
Dexib® 100.12% ±  0.51 97.18% ±  0.38 99.95% ±  0.45 100.50% ±  0.91
Zibufen® 99.43 % ±  0.12 98.72% ±  0.43 100.35% ±  0.25 97.65% ±  0.83

SD = Standard Deviation.

Table 3: Contents of DBPN, SBZ, MP and PP in the fixed dose combination suspension.

The statistical analysis showed no significant difference between results obtained employing the analytical conditions established for the method and those obtained in the experiments in which variations of some parameters were introduced. The parameters used in system suitability test were symmetry of peaks, tailing factor, resolution and RSD of peak area for replicate suspension. Thus, the method showed to be robust for changes in mobile phase Acetonitrile proportion, mobile phase pH, flow rate, and column temperature (Table 4).

Chromatographic Conditions Variation Retention time
DBPN SBZ MP PP
Temperature (°C) 22 15.781 3.337 4.235 7.543
30 17.019 3.598 4.712 8.135
Flow rate (ml/min.) 0.8 20.949 4.426 5.842 9.936
1.2 13.960 2.937 3.882 6.638
Amount of Acetonitrile (%) 48 15.972 3.543 4.566 7.782
52 15.398 2.897 3.959 7.165

Table 4: Robustness of the method.

Stability testing is an important part of the process of drug product development. The purpose of stability testing is to provide evidence of how the quality of a drug substance or drug product varies with time under a variety of environmental conditions, for example temperature, humidity, and light and enables recommendation of storage conditions, retest periods, and shelf life to be established. The two main aspects of drug product that play an important role in shelf-life determination are assay of the active drug and the degradation products generated during stability studies. The proposed assay method was applied to stability study of commercially available suspension, for which the samples were placed at 30°C with relative humidity of 65% and at 40°C with relative humidity of 75%. Stability study was performed according to stability protocol as described in previous section (Table 5). Samples were analyzed and percentage of contents was measured. According to the results obtained DBPN with all the three preservatives were found to be stable at applied conditions of temperature and relative humidity, and were accurately analyzed with the proposed method.

TEST (Claimed content) Interval Mean RSD%
Initial 1Month 2Month 3Month 4Month 5Month 6Month
Studies at Accelerated (40°C+75%H)
DBPN (500 µg/ml) 100.351 99.961 99.476 99.265 98.971 98.432 98.157 99.044 0.794
SBZ (12.5 µg/ml) 99.853  98.983  98.418  98.137  97.975  97.869 97.535 98.153 0.803
MP (5 µg/ml) 99.991 98.615 98.315 98.158 97.518 97.410 97.112 98.160 0.989
PP (2.5 µg/ml) 99.614 99.741 99.534 98.614 98.198 97.536 97.418 98.665 1.002
Stability Studies at Long Term  (30°C+65%H)
DBPN (500 µg/ml) 100.351 100.135 100.384 100.174 99.853 99.239 99.472 99.876 0.443
SBZ (12.5 µg/ml) 100.516 99.623 99.398 98.938 98.917 98.374 98.126 98.896 0.644
MP (5 µg/ml) 101.219 100.526 100.153 99.315 98.815 98.345 98.151 99.503 1.167
PP (2.5 µg/ml) 101.648 101.689 100.235 99.958 99.461 98.432 98.012 99.919 1.432

Table 5: Summary of stability studies.

During the degradation study upon treatment of DBPN, SBZ, MP and PP with base (0.1M NaOH), acid (0.1M HCl), hydrogen peroxide (35%) and heat it was observed that DBPN with preservatives is fairly stable to acid and heat (Figure 4 and 5). Esters of MP, PP and salt of carboxylic acid showed thermally stability and no decarboxylation occurs to DBPN up to this temperature. Also with dilute mineral acid most suspected reaction of sodium benzoate by reforming carboxylic acid could be possible but shows no peculiar change in absorbance pattern of its chromophore. The data in Table 6, also revealed that SBZ and DBPN are usually more vulnerable to the stress conditions as compared to MP and PP. Further in Figure 6, base treated sample, degraded peaks of DBPN & SBZ is the Clear evidence of base hydrolysis of carboxylic group [38]. Extremely high absorbance SBZ in peroxide treated sample (Figure 7) showing the sensitivity of carboxylic salt being oxidized.

pharmaceutica-analytica-acta-heat

Figure 4: Typical chromatogram of heat treated sample.

pharmaceutica-analytica-acta-acid

Figure 5: Typical chromatogram of acid treated sample.

pharmaceutica-analytica-acta-base

Figure 6: Typical chromatogram of base treated sample.

pharmaceutica-analytica-acta-base

Figure 7: Typical Chromatogram of H2O2 treated sample.

Stress conditions Time/h Assay of active Substance Degradation (%)
DBPN SBZ MP PP DBPN SBZ MP PP
Acid hydrolysis (0.1M HCl) 1 96.20% 99.55% 99.71% 98.97% 3.30% 0.45% 0.29% 1.03%
Base hydrolysis (0.5M NaOH) 1 99.57%* 99.93%* 96.49% 98.51% 0.43%* 0.07%* 3.51% 1.49%
Thermal (60°C) 1 96.20% 99.55% 99.71% 98.97% 3.80% 0.45% 0.29% 1.03%
Oxidative hydrolysis (35% H2O2) 1 80.48% 84.76% 113.58% 94.94% 19.52% 15.24% 13.58% 5.06%

Table 6: Summary of forced degradation results.

Conclusion

The proposed new HPLC method described in this paper provides a simple, convenient and reproducible approach for the simultaneous identification and quantification of Dexibuprofen, Sodium Benzoate, Methylparaben and Propylparaben in bulk, human serum, and pharmaceutical formulations with good separation and resolution. In addition, this method has the potential application to clinical research of drug combination. Analytical results are accurate and precise with good recovery and lowest detection limit values. In short, the developed method is simple, sensitive, easy, and efficient having short chromatographic time and can be used for routine analysis in QC laboratory and therapeutic monitoring.

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Citation: Hasan N, Chaiharn M, Shah SN, Khalid H, Jabbar A (2013) Simultaneous Determination of NSAID and Antimicrobial Preservatives Using Validated RPHPLC Method: An Application in Pharmaceutical and Clinical Laboratories. Pharm Anal Acta 4:263.

Copyright: © 2013 Hasan N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.