Significant Improvements in the Performance of an Established Affinity Chromatography Procedure Employed to Purify a Monoclonal Antibody in 100 Purification Cycles

Williams Ferro¹, Rodolfo Valdés^1*, Eutimio Fernández², Yarysel Guevara¹, Yenisley Medina¹, Tatiana Álvarez¹, Andrés Tamayo¹, Tatiana González³, Mayra Wood³, Maylín La O⁴, Yodelvis Calvo⁴ and Déborah Geada⁵

¹Monoclonal Antibody Department, Center for Genetic Engineering and Biotechnology, Ave 31/ 158 and 190, P.O. Box 6162, Havana 10600, Cuba

²Nuclear and Energy Research Institute, Ave. Lineu Prestes, 2242, Cidade Universitaria, Butantã, Sao Paulo, 05508000, Brazil

³Process Control Department, Center for Genetic Engineering and Biotechnology, Ave 31/ 158 and 190, P.O. Box 6162, Havana 10600, Cuba

⁴Quality Control Department, Center for Genetic Engineering and Biotechnology, Ave 31/ 158 and 190, P.O. Box 6162, Havana 10600, Cuba

⁵Tobacco Research Institute, San Antonio de los Baños, Havana 3500, Cuba