Journal of Carcinogenesis & Mutagenesis

Knowing the Mechanisms: How Probiotics Affect the Development and Progression of Cancer

Abhinandan Patil^1*, Devika Kotekar² and Ghanshyam Chavan^{3 1}Department of Pharmaceutics, D.Y. Patil Education Society (Deemed to be University), Kolhapur, India
²Department of Pharmaceutics, Maratha Mandal College of Pharmacy, Belgaum, India
³Department of Pharmaceutics, SVS’s Dadasaheb Rawal Pharmacy College, Dondaicha, India
^*Correspondence: Abhinandan Patil, Department of Pharmaceutics, D.Y. Patil Education Society (Deemed to be University), Kolhapur, India, Email:

Received: 19-Jul-2023, Manuscript No. JCM-23-22258; Editor assigned: 21-Jul-2023, Pre QC No. JCM-23-22258(PQ); Reviewed: 04-Aug-2023, QC No. JCM-23-22258; Revised: 11-Aug-2023, Manuscript No. JCM-23-22258(R); Published: 18-Aug-2023, DOI: 10.35248/ 2157-2518.23.14.421

Introduction

Gut Microbiota Modulation is the ability of probiotics to change the make-up and function of the gut microbiota, which is the group of microorganisms that live in a person's digestive system. This subtopic is about learning how probiotics can change the balance of bacteria, fungus, viruses, and other microorganisms in the gut and how these changes can affect the growth and spread of cancer.

The gut microbiota is very important for keeping general health. It helps control the immune system, digest food, absorb nutrients, and even keep your mind healthy. New study shows that dysbiosis, which is an imbalance or change in the gut microbiota, may be linked to many diseases, including cancer [1-3].

Probiotics are live bacteria that help the health of the person when they are eaten in the right amounts. They can add good bacteria to the gut flora or help the good bacteria that are already there grow and work better. By doing this, probiotics can help establish a healthy balance in the bacteria of the gut and help it work well.

In terms of cancer, gut flora regulation by probiotics is being looked into to see if it could affect how cancer starts and grows. Some studies have shown that certain types of probiotics can help the body's immune system fight cancer cells, stop tumors from growing, and even make cancer treatments like chemotherapy and immunotherapy work better. Also, it has been found that the gut bacteria makes chemicals that can either help or hurt the growth of cancer cells. Probiotics can change the way these metabolites are made, which could make the climate less friendly for cancer cells or decrease the production of harmful chemicals that can lead to tumors. Understanding how probiotics change the gut microbiota and how that affects the growth and spread of cancer is important for coming up with new ways to avoid and treat cancer. More study is needed to find out which probiotic types work best against cancer, how much of them to take, and what molecular processes are at work [4-7].

Literature Review

Relationship between probiotics, gut microbiota and the growth and spread of cancer

More study is needed to find out which probiotic types work best against cancer, how much of them to take, and what molecular processes are at work.

Immune system modulation: Researchers are looking into how probiotics affect the immune system to improve the immune system's response to cancer cells and possibly stop tumor growth. Immune System Modulation is the ability of probiotics to work with and change the immune system. This is especially true when it comes to boosting the immune system's reaction to cancer cells and possibly stopping tumor growth. This subtopic looks at how probiotics and the immune system work together in the fight against cancer. The immune system is very important for finding and getting rid of abnormal cells, like cancer cells. But cancer cells can find ways to avoid being found and killed by the immune system. Researchers are looking into how probiotics can change the immune system to make it better able to find and kill cancer cells and stop tumors from growing. Immune cells like lymphocytes, macrophages, and dendritic cells can interact with probiotics in different ways [8-12]. They can cause the body to make more immunity-regulating chemicals, like cytokines and chemokines, which control how immune cells work and talk to each other. Probiotics can also make Natural Killer (NK) cells work better and make more of them. NK cells are specialized immune cells that play a key role in finding and killing cancer cells. Probiotics can also change how the immune system's proinflammatory and anti-inflammatory reactions work together [13-15]. Cancer is more likely to start or get worse if there is a lot of chronic inflammation. Probiotics have been shown to help the immune system work better by calming down too much inflammation and boosting anti-inflammatory messages. This makes it hard for cancer cells to grow. In animal models, studies have shown that certain types of probiotics can help the immune system fight cancer cells better. They have been shown to increase the number of immune cells that attack tumors, boost the killing activity of immune cells, and encourage the development of a memory response that can protect against cancer coming back for a long time. But it's important to remember that the immune system's reaction to cancer is complicated and has many parts. Also, how probiotics and the immune system work together in the setting of cancer is still being studied. More research is needed to figure out how probiotics change the immune system's reaction to cancer, find the most effective probiotic strains, and figure out the best doses for getting therapeutic effects [16-21].

Probiotics affecting the immune system in setting of cancer

Anti-inflammatory effects: This section looks at how probiotics reduce inflammation, which is closely linked to the growth of cancer.

Anti-inflammatory effects relate to probiotics' ability to reduce inflammation, which is closely linked to the development of cancer. This subtopic looks at how probiotics can change the way the body responds to inflammation and could possibly help prevent or treat cancer. Inflammation is a normal way for the body's immune system to protect itself from dangerous things like infections or damaged tissues. But inflammation that lasts for a long time can help cancer start and spread. It makes an environment that encourages cell growth, genetic changes, and the growth of new blood vessels, all of which are signs of cancer [22-26].

Researchers have looked into how probiotics might be able to control inflammation by reducing it. They can change the balance between pro-inflammatory and anti-inflammatory messages, which helps reduce inflammation that lasts too long or is too strong. One way that probiotics reduce inflammation is by making things like Short-Chain Fatty Acids (SCFAs). SCFAs are waste products that are made when probiotics react in the gut. They have been shown to stop the production of proinflammatory cytokines and increase the release of antiinflammatory cytokines. This changes the immune reaction to be less inflammatory. Probiotics can also improve the performance of the gut barrier, which is important for stopping dangerous substances from getting into the bloodstream from the gut. When the barrier between the gut and the rest of the body is broken, it can cause systemic inflammation, which can lead to many illnesses, including cancer. Probiotics help keep the gut barrier strong, which makes it harder for pro-inflammatory molecules to get into the bloodstream and reduces the body's inflammatory reaction (Table 1). Probiotics can also work with immune cells like macrophages and dendritic cells, changing how they become active and causing them to send out more anti-inflammatory messages. They can also stop the activity of Nuclear Factor-Kappa B (NF-B), which is a key transcription factor in how inflammation is controlled [27-31].

Table 1: Impact of probiotics in different health conditions

Probiotics may make it harder for cancer to grow and spread by reducing chronic inflammation. Even though more research is needed to fully understand the exact mechanisms and best probiotic strains, several animal and in vitro studies have shown promising results, which suggests that probiotics could be used to help avoid and treat cancer. Even though probiotics may be able to control inflammation and lower the risk of cancer, they should not be thought of as a cure for cancer on their own. Along with standard cancer treatments and healthy living choices, they can be thought of as part of a complete plan to lower inflammation and improve general health [32-34].

Metabolism and metabolites: Knowing how probiotics affected by apoptosis, cell cycle control, and angiogenesis. Metabolism and metabolites are terms for the ways that probiotics change how the body uses nutrients and make chemicals that can change how cancer cells act. This subtopic looks at how probiotics can change metabolism and make bioactive molecules that affect important parts of cancer cell biology, such as apoptosis (cell death), cell cycle control, and angiogenesis (formation of new blood vessels).

Probiotics can change the way nutrients are used in many ways. They can make it easier for the body to digest and absorb things like carbohydrates, proteins, and fats, which leads to better use of nutrients. Probiotics can also make enzymes that break down complicated molecules, which makes them easier for the body to absorb and use.

As probiotics break down nutrients, they make byproducts like Short-Chain Fatty Acids (SCFAs), vitamins, polyamines, and other bioactive chemicals. These molecules can change how cancer cells act in different ways. SCFAs, such as butyrate, propionate, and acetate, are an important group of chemicals that are made by bacteria. SCFAs are important for keeping the intestines healthy and have been shown to change the way cancer cells act. For example, butyrate has been shown to cause apoptosis, stop cancer cells from growing, and control how the cell cycle goes. SCFAs can also change the immune reaction, improve the function of the gut barrier, and lower inflammation. All of these things can affect how cancer starts and grows. Some vitamins, like folate, B vitamins, and vitamin K, are made by probiotics. These vitamins are important for the health of cells and the body as a whole. These vitamins help make, fix, and control DNA, and the amount of them in the body can change how cancer cells act [1-3,15].

Probiotics also make polyamines, which are a type of substance. They are involved in cell growth, cell division, and cell death. Probiotics can change how much polyamines are made and how much is available. This may affect how cancer cells grow and live.

Also, probiotics can make other useful molecules like bacteriocins and exopolysaccharides, which have shown that they might be able to fight cancer. Bacteriocins are antimicrobial peptides that are made by probiotics. They can stop the growth of harmful bacteria, like those that can lead to cancer, by stopping the production of new proteins. Exopolysaccharides made by bacteria have been shown to affect the immune system and fight cancer [8].

Probiotics effect on metabolism of cancer cells

It gives some ideas about how probiotics might affect the growth, spread, and treatment of cancer. But it's important to remember that different probiotic types and their products may have different effects on cancer cells. More research needs to be done to figure out the exact processes and find the best strains and metabolites for specific cancer treatments.

Gut barrier integrity: Looking at how probiotics affect the operation of the intestinal barrier, stop the movement of harmful substances, and possibly lower the risk of cancer in the digestive system.

Gut Barrier Integrity is the ability of probiotics to keep the gut barrier in good shape and help it work well. This subtopic is about learning how probiotics can improve the gut barrier, stop dangerous substances from getting into the bloodstream from the gut, and possibly lower the risk of developing cancer in the GI tract [11,33].

The intestinal barrier is a physical and chemical barrier that keeps the contents of the gut, such as germs and their waste, from getting into the tissues and bloodstream below. When the gut barrier is broken, it can lead to greater permeability. This means that harmful substances can get through and cause inflammation and immune reactions, which could lead to diseases like cancer.

Probiotics have been shown to improve the health of the gut barrier. They can help strengthen the tight links between intestine cells, which are what keep the barrier from breaking down. Probiotics help the body make proteins that make these tight junctions. This makes it harder for dangerous chemicals to get through the gut lining.

Probiotics can also help the body make more mucins. Mucins are protective proteins that form a physical layer on the surface of the intestines. Mucins help keep the gut epithelial cells from coming into direct touch with the luminal fluids, which can contain dangerous bacteria and their toxins. Probiotics can help keep the health of the gut barrier by making the body make more mucus.

Also, probiotics can change the make-up and balance of the gut flora, which is an important part of how the gut barrier works. Certain dangerous bacteria or an imbalance in the gut flora (dysbiosis) can damage the gut barrier and raise the risk of inflammation, disease, and even cancer. Probiotics can help restore a healthy balance of gut bacteria by stopping the growth of dangerous bacteria and encouraging the growth of good bacteria. This may help maintain the integrity of the gut barrier [11,33].

Probiotics help keep the gut barrier strong so that dangerous substances like bacteria, toxins, and chemicals that cause inflammation don't get into the bloodstream. This can lower the risk of cancer in the gut by reducing overall inflammation and the chance that harmful substances will get into the stomach tract [14].

Even though more research is needed to figure out the link between a healthy gut barrier, probiotics, and preventing cancer, studies in both experimental and clinical settings have shown hopeful results. Understanding how probiotics affect the health of the gut barrier and how they might lower the risk of gastrointestinal cancers can help come up with new ways to avoid and treat cancer.

Conclusion

In conclusion, the fact that probiotics can change the integrity of the gut barrier is a hopeful way to lower the risk of cancer in the GI system. Probiotics have been shown to help keep a strong gut barrier by strengthening tight junctions, increasing mucin production, and restoring a healthy balance in the gut flora. By stopping dangerous substances from getting into the bloodstream, probiotics may reduce inflammation and protect the GI tract from the effects of these substances that could cause cancer. Even though study is still going on in this area, what we know so far says that probiotics could be used as an additional way to prevent cancer. Future studies should dig deeper into figuring out the exact processes and figuring out which probiotic strains work best to improve the health of the gut barrier and lower the risk of gastrointestinal cancers. By using the healing power of probiotics to improve the health of the gut barrier, we may be able to find new ways to avoid and treat cancer in the future.

References

1. Afzal M, Mazhar SF, Sana S, Naeem M, Rasool MH, Saqalein M, et al. Neurological and cognitive significance of probiotics: A holy grail deciding individual personality. Future Microbiol. 2020;15:1059-1074.

   [Cross Ref] [Google Scholar] [Pub Med].

2. Markowiak P, Slizewska K. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients. 2017.

   [Cross Ref] [Google Scholar] [Pub Med]

3. Kazemian N, Mahmoudi M, Halperin F, Wu JC, Pakpour S. Gut microbiota and cardiovascular disease: Opportunities and challenges. Microbiome. 2020;8(1):36.

   [Cross Ref] [Google Scholar] [Pub Med]

4. Hills RD Jr, Pontefract BA, Mishcon HR, Black CA, Sutton SC, Theberge CR. Gut microbiome: Profound implications for diet and disease. Nutrients. 2019.

   [Cross Ref] [Google Scholar] [Pub Med]

5. Panebianco C, Andriulli A, Pazienza V. Pharmacomicrobiomics: Exploiting the drug-microbiota interactions in anticancer therapies. Microbiome. 2018;6(1):92.

   [Cross Ref] [Google Scholar] [Pub Med].

6. Khan AA, Shrivastava A, Khurshid M. Normal to cancer microbiome transformation and its implication in cancer diagnosis. Biochem Biophys Acta. 2012;1826(2):331–337.

   [Cross Ref] [Google Scholar] [Pub Med].

7. Khan AA, Nema V, Khan Z. Current status of probiotics for prevention and management of gastrointestinal cancers. Expert Opin Biol Therapy. 2020.

   [Cross Ref] [Google Scholar] [Pub Med].

8. Khan AA, Khurshid M, Khan S, Alshamsan A. Gut microbiota and probiotics: Current status and their role in cancer therapeutics. Drug Dev Res. 2013;74(6):365–375.

   [Cross Ref] [Google Scholar]

9. Sedighi M, Bialvaei AZ, Hamblin MR, Ohadi E, Asadi A, Halajzadeh M, et al. Therapeutic bacteria to combat cancer; current advances, challenges, and opportunities. Cancer Med. 2019;8(6):3167-3181.

   [Cross Ref] [Google Scholar] [Pub Med].

10. Zur HH. The search for infectious causes of human cancers: Where and why. Virology. 2009;392(1):1–10.

    [Cross Ref] [Google Scholar] [Pub Med].

11. Ishaq S, Nunn L. Helicobacter pylori and gastric cancer: A state of the art review. Gastroenterol Hepatol Bed Bench. 2015;8:S6–S14.

    [Google Scholar] [Pub Med]

12. Khan AA, Abuderman AA, Ashraf MT, Khan Z. Protein-protein interactions of hpv-chlamydia trachomatis-human and their potential in cervical cancer. Future Microbiol. 2020;15:509–520.

    [Cross Ref] [Google Scholar] [Pub Med].

13. Khan AA, Khan Z, Malik A, Kalam MA, Cash P, Ashraf MT, et al. Colorectal cancer-inflammatory bowel disease nexus and felony of Escherichia coli. Life Sci. 2017;180:60–67.

    [Cross Ref] [Google Scholar] [Pub Med].

14. Khan AA, Khan Z, Malik A, Shrivastava A, Jain SK, Alshamsan A. Computational prediction of Escherichia coli proteins targeting the host cell nucleus, with special reference to their role in colon cancer etiology. Cancer Lett. 2015;364(1):25–32.

    [Cross Ref] [Google Scholar] [Pub Med].

15. Khan AA. In silico prediction of Escherichia coli proteins targeting the host cell nucleus, with special reference to their role in colon cancer etiology. J Comput Biol. 2014;21(6):466–475.

    [Cross Ref] [Google Scholar] [Pub Med].

16. Khan AA, Cash PE. E.coli and colon cancer: Is mutY a culprit? Cancer Lett. 2013;341(2):127–131.

    [Cross Ref] [Google Scholar] [Pub Med].

17. Tewari M, Mishra RR, Shukla HS. Salmonella typhi and gallbladder cancer: Report from an endemic region. Hepat Pancreat Dis Int HBPD INT. 2010;9(5):524–530.

    [Pub Med] [Google Scholar]

18. Sheflin AM, Whitney AK, Weir TL. Cancer-promoting effects of microbial dysbiosis. Curr Oncol Rep. 2014;16(10):406.

    [Cross Ref] [Google Scholar] [Pub Med].

19. Jenkins SV, Robeson MS 2nd, Griffin RJ, Quick CM, Siegel ER, Cannon MJ, et al. Gastrointestinal tract dysbiosis enhances distal tumor progression through suppression of leukocyte trafficking. Cancer Res. 2019;79(23):5999–6009.

    [Cross Ref] [Google Scholar] [Pub Med].

20. Gao Z, Guo B, Gao R, Zhu Q, Qin H. Microbiota disbiosis is associated with colorectal cancer. Front Microbiol. 2015;6:20.

    [Cross Ref] [Google Scholar] [Pub Med].

21. Xuan C, Shamonki JM, Chung A, Dinome ML, Chung M, Sieling PA, et al. Microbial dysbiosis is associated with human breast cancer. PLoS ONE. 2014;9(1):e83744.

    [Cross Ref] [Google Scholar] [Pub Med].

22. Malla MA, Dubey A, Kumar A, Patil A, Ahmad S, Kothari R, et al. Optimization and elucidation of organophosphorus and pyrethroid degradation pathways by a novel bacterial consortium C3 using RSM and GC-MS-based metabolomics. Journal of the Taiwan Institute of Chemical Engineers. 2021;144:104744.

    [Google Scholar]

23. Munot N, Kandekar U, Rikame C, Patil A, Sengupta P, Urooj S, et al. Improved mucoadhesion, permeation and in vitro anticancer potential of synthesized thiolated acacia and karaya gum combination: A systematic study. Molecules. 2022;27(20):6829.

    [Cross Ref] [Google Scholar] [Pub Med]

24. Munot N, Kandekar U, Giram PS, Khot K, Patil A, Cavalu S. A comparative study of quercetin-loaded nanocochleates and liposomes: Formulation, characterization, assessment of degradation and in vitro anticancer potential. Pharmaceutics. 2022;14(8):1601.

    [Cross Ref] [Google Scholar] [Pub Med]

25. Manikyam HK, Tripathi P, Patil SB, Lamichhane J, Chaitanya M. Extraction, purification, and quantification of hesperidin from the immature Citrus grandis/maxima fruit Nepal cultivar. Asian J Nat Product Biochem. 2020;20(1).

    [Google Scholar]

26. Patil A, Munot N, Patwekar M, Patwekar F, Ahmad I, Alraey Y, et al. Encapsulation of lactic acid bacteria by lyophilisation with its effects on viability and adhesion properties. Evid Based Complement Alternat Med. 2022.

    [Cross Ref] [Google Scholar] [Pub Med]

27. Nalawade AS, Gurav RV, Patil AR, Patwekar M, Patwekar F. A comprehensive review on morphological, genetic and phytochemical diversity, breeding and bioprospecting studies of genus Chlorophytum Ker Gawl. from India. Trends Phytochem Res. 2022;6(1):19-45.

    [Cross Ref] [Google Scholar]

28. Patil KG, Balkundhi S, Joshi H, Ghewade G. Mehsana buffalo milk as prebiotics for growth of lactobacillus. International Journal of Pharmacy and Pharmaceutical Research. 2011;1(1):114-117.

    [Google Scholar]

29. Das N, Ray N, Patil AR, Saini SS, Waghmode B, Ghosh C, et al. Inhibitory effect of selected Indian honey on colon cancer cell growth by inducing apoptosis and targeting the β-catenin/Wnt pathway. Food Funct. 2022;13(15):8283-8303.

    [Cross Ref] [Google Scholar] [Pub Med]

30. Patil MJ, Mali V. The diverse cytotoxicity evaluation of lactobacillus discovered from sheep milk. Acta Sci Pharmac Sci. 2021;5(12):69-70.

    [Google Scholar]

31. Abhinandan P SP, John D. Probiotic potential of Lactobacillus plantarum with the cell adhesion properties. Journal of Global Pharma Technology. 2020;10(12):1-6.

    [Google Scholar]

32. Patil A, Pawar S, Disouza J. Granules of unistrain lactobacillus as nutraceutical antioxidant agent. Intern J pharmaceut Sci Res. 2018;9(4):1594-1599.

    [Google Scholar]

33. Patil A, Mali V, Patil R. Banana fibers camouflaging as a gut worm in a 6-month-old infant. Iberoam J Med. 2019;2(3):245-247.

    [Cross Ref] [Google Scholar]

34. Pawar V, Patil A, Tamboli F, Gaikwad D, Mali D, Shinde A. Harnessing the power of AI in pharmacokinetics and pharmacodynamics: A comprehensive review. AAPS PharmSciTech. 2023;14(2),426-439.

    [Google Scholar]