Journal of Biomolecular Research & Therapeutics

Ravi Vattepu

Ravi Vattepu
Researcher, Center for Immunology and Inflammatory Diseases, Harvard Medical School, Massachusetts General Hospital
USA

Biography

• PhD in Chemistry, Wichita State University, Wichita, Kansas, USA 2012-2017 “Investigating the role of actin binding protein palladin in actin regulationâ€Â • M.S. Organic Chemistry Osmania University, Hyderabad, India 2008 - 2010 • B.S. Bio-Technology, Chemistry Osmania University, Hyderabad, India 2005 – 2008

Research Interest

Over-expression and purification of proteins in bacterial, mammalian expression system and metabolic isotopic labeling (15N or 15N, 13C,) of proteins for protein NMR. Protein purification using varied chromatography techniques (affinity, ion-exchange and size-exclusion). Biophysical characterization of proteins and protein-ligand interactions using fluorescence Spectroscopy, Pull down assays, Bio Layer Interferometry (BLI) and Isothermal Titration Calorimetry (ITC)  Structural characterization of protein by Circular Dichroism Spectroscopy (CD), fluorescence Spectroscopy. Antibody glyco engineering and biophysical characterization of antibody interactions using SPR. Antibody and FcR receptor binding and signaling pathway investigation  Antibody induced inflammation using mouse models  Probing protein conformational changes using FRET, Pulse Proteolysis, and Chemical Cross-linking. Quantification of Thiol-Content in the protein.  Extensive experience Protein labeling with Fluorophore (N-(1-Pyrenyl)maleimide, monoBromobimane)  Actin structural network is observed using Confocal microscopy, Leica TCS SP5 instrument (used to observe effect of palladin on actin organization). Molecular biology techniques Cloning, Western Blot, Far Western Blot, and Cell Culture. Software usage Graph pad prism / Image J and End-note. Expertise in handling breast cancer tissue and Isolated tumor cells for the cell invasion assay. Ability to develop processes