Journal of Bacteriology & Parasitology

Konttinen T

Konttinen T
Professor, Department of Medicine
University of Helsinki, Finland

Biography

Dr. T. Konttinen has specialized in Internal Medicine and Rheumatology.
Visiting researcher at UCSD with Professor Nathan J. Zvaifler and Professor Harry G. Bluestein from 1982 to 1984 and NYU with Professor Robert J. Winchester from 1989 to 1991. He was a Professor of Oral Medicine at the University of Helsinki, Faculty of Dentistry from 1999 to 2003.
He is now Professor of Medicine and Chief Physician at the Hospital District of Helsinki and Uusimaa, Internal Medicine. He is also a Research Director at Orton Foundation Research Institute and COXA Hospital for the Joint Replacement. He has published 614 papers enlisted in PubMed, Hirsch index 50. He has supervised 34 PhD thesis works, peer reviews for 93 different journals, 151 invited lectures abroad, grant evaluations for 25 international organizations. He Co-operates with internal and external researchers and research groups.

Research Interest

Cell culture and stimulations are much used, but also clinical and experimental tissue samples are analyzed. BOND-MAX, LEICA is fully automated staining system for frozen and paraffin embedded specimens to localize specific proteins (antigen) in tissues or cell culture specimen by immunohistochemcal staining. In situ hybridisation technique is in use to localize a specific DNA or RNA sequence in a specimen. Light microscopy is the routine method to visualize results of IHC and ISH staining while confocal laser scanning microscopy is a tool for imaging cells and tissues three-dimensionally.
Western-blot and Enzyme-Linked Immunosorbent Assay, ELISA methods are used to detect proteins, which can be quantified by ProEXPRESS imaging system.
In molecular biology protein production, cloning and gene expression studies are in use. Quantitative real time polymerase chains reaction (qPCR) is in use to amplify and quantification of a specific sequence on a sample. Our equipment consist of IQ™5, Multicolour Real-Time PCR detection System,(Bio-Rad) and Light Cycler, (Roche).
Multiplex analysing assay system, which is soon coming to use, will enable us to make microRNA analysis, immunoassays, nucleic acid hybridization assays etc from a wide range of samples. With this method we will be able to assay phosphorylation states and expression levels of multiple proteins and make highly sensitive cytokine assays.