Journal of Allergy & Therapy

Immunology Discovery, Abbvie Inc., North Waukegan Road, North Chicago, IL 60064, USA

Publications

• Review Article   
  Cellular, Molecular, and Pharmacological Characterization of a House Dust Mites (HDM)-Induced Murine Model of Dermatitis
  Author(s): Nancy Crosbie, Jacqueline Loud*, Victoria E. Scott, Viktor TodoroviÄ?, Ramkrishna Sadhukhan, Heath A. McDonald, Danyal Butt, Samantha Ciura, Stephanie E. Paulsboe, Gricelda Simler, Elizabeth Asque, Laura Wasserman, Zhi Su, Gunarso Nguyen, Samuel Karsen, Joseph B. Wetter, Anastasia E. Marinopoulos, Stacy H. Ryu, Xin Chen, Kathleen M. Smith, Sheila Cummings, Zachary K. Goldsmith and Samrawit Aforki
  
  Background: House Dust Mites (HDM), common allergens that can induce Atopic Dermatitis (AD), are widely employed to generate mouse models of AD. In the current study, we compared the AD-like phenotypes between two mouse strains, NC/Nga, and BALB/c, in response to HDM, and performed cellular, molecular, and pharmacological characterization of HDM-induced dermatitis in NC/Nga mice. Methods: In-life endpoints (skin clinical scores, ear thickness, Transepithelial Water Loss (TEWL), scratching bouts) and terminal endpoints (histopathology, total serum IgE and tissue cytokines) were measured. Further phenotyping of NC/Nga was performed by flow cytometry, gene expression analysis, and pharmacology. Results: HDM applications resulted in a more robust AD-like dermatitis in NC/Nga than BALB/c mice as evidenced by greater cha.. View more»
  

  Abstract PDF