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Yetunde M Feruke-Bello
Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
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Research Article
Bioremediation Potential of Heavy Metal Multi-Tolerant Pseudomonas Spp. Isolated From a Municipal Waste Dumpsite at Ile-Ife, Osun State, Nigeria
Author(s): Yetunde M Feruke-Bello*, Olu Odeyemi and Gbolahan O Babalola
Municipal wastes are probable source of heavy metals that may be noxious to the environment and the biotic component due to indiscriminate waste disposal method and open waste dump practiced in Africa. Three Pseudomonas spp. isolated from a municipal waste dumpsite were evaluated for their tolerance to five metals (Pb, Cd, As, Ni and Cr). The best isolate that showed multiple resistance to all the five metals was identified as Pseudomonas aeruginosa by the 16S rRNA sequence with a Maximum Tolerable Concentrations (MTC) of 2000 mg/l for Pb, 1200 mg/l for Cd, 4500 mg/l for As, 600 mg/l for Ni and 2000 mg/l for Cr. Pseudomonas aeruginosa was subjected to mutation by ethidium bromide and acridine orange; thirteen mutant strains were recovered. The Pseudomonas aeruginosa MutAb, MutAc and MutAe exhibited a 25% increase in the MTC to lead.. View more»