Clinical & Medical Biochemistry

Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Biography

Rajni Sharma works in the Department of Experimental Medicine and Biotechnology and graduated from Post Graduate Institute of Medical Education and Research in the  Chandigarh in  India Rajni Sharma International experience includes various programs, contributions and participation in different countries for diverse fields of study.

Publications

• Research Article   
  Epigenetic Modifications of Nucleotide Excision Repair Genes in Oral Squamous Cell Carcinoma
  Author(s): Cynthia Cyriac, Rajni Sharma, Gursonika Binepal, Naresh Panda and Madhu Khullar*
  
  Genotoxic exposure to tobacco carcinogens resulting in DNA damage is an important mechanism of oral squamous cell carcinoma (OSCC) etiology. Nucleotide Excision Repair (NER) pathway removes bulky DNA adducts, generated from tobacco exposure thereby playing a major role in initiation of OSCC. In addition to mutations, epigenetic modifications have also been shown to target DNA repair genes thereby modulating oral tumor genesis. We therefore examined the role of epigenetic alterations modulating expression of three NER genes; XPC, XPB and XPD involved in removal of adducts caused by major classes of tobacco carcinogens and their contribution to OSCC Methylation status of NER genes was assessed using methylation specific PCR (MSP) in biopsies taken from 52 OSCC patients, their surrounding margins and 27 normal controls. The mRNA levels were determined using quantitative real time PCR (qRT-P.. View more»
  

  DOI: 10.4172/2471-2663.1000103

  Abstract PDF