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Antonio Antico
Department of Laboratory Medicine, ULSS7 Pedemontana, Santorso, Vicenza, Italy
Publications
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Research Article
Is the Interaction of Technology useful in Laboratory Haematology Diagnostics?
Author(s): Alessandra Falda*, Marco Falda, Aurelio Pacioni, Giada Borgo, Rosolino Russelli and Antonio Antico
Background: Monoclonal B lymphocytosis (MBL) increases with age and individuals with high count MBL progress to chronic lymphocytic leukaemia requiring therapy at a rate of ~1%-5% per year. These cases usually have atypical lymphocytes at the microscope, abnormal representation in the scattergram, and positivity of flags. Using XN9000 (Sysmex), we noticed cases of MBL without this correlation. We studied customized gates for discovering MBL cases of our interest. Methods: We considered 212 peripheral blood samples with known phenotypes: 76.7% negative and 23.3% positive for B, T, or NK lymphocytes clones. We created gates studying the XN9000 FCS files in Diva software to identify new areas for better delimiting subpopulations of our interest and calculating sensitivity and specificity. Results: We fou.. View more»