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AM Soliman

Department of Plant Virus and Phytoplasma Research, Plant Pathology Research Institute, Agricultural Research Center, Cairo, Egypt

  • Research Article   
    Relationship of Luteovirus and PAV-Barley Yellow Dwarf Virus
    Author(s): Hoda MA Waziri*, Hala A Amin, KE Saker and AM Soliman

    Barley Yellow Dwarf Virus (BYDV-PAV) isolated from Wheat plants grown in Egypt has been characterized. Two coding regions of the Barley Yellow Dwarf Virus (BYDV-PAV) isolate for the polymerase gene (P1) located in Open Reading Frame (ORF1) and Coat Protein gene (CP) located in Open Reading Frame (ORF3) was targeted. Two sets of specific primers were designed according to of BYDV-PAV isolate in Genbank. The DNA fragments of ORF1and ORF3 of an Egyptian isolate of BYDV-PAV were cloned and sequenced. The sequence contained a full-length ORF1 coding for the viral polymerase gene (P1). It comprises 910 knot in length encodes a predicted polypeptide chain of 303 amino acids with an M(r) of 34.67. On the other hand, the sequence data for the ORF3 coding for the viral coat protein revealed that it was 603 bp in length encodes a predicted protein 200 amino acids, with molecular weight of 21.96 .. View more»

    DOI: 10.35248/2157-7471.22.13.629

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