Journal of Microbial & Biochemical Technology

Yoshito Ando

Publications

• Research Article
  Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172
  Author(s): Lian-Ngit Yee, Tabassum Mumtaz, Mitra Mohammadi, Lai-Yee Phang, Yoshito Ando, Abdul Rahim Raha, Kumar Sudesh, Hidayah Ariffin, Mohd Ali Hassan and Mohd Rafein ZakariaLian-Ngit Yee, Tabassum Mumtaz, Mitra Mohammadi, Lai-Yee Phang, Yoshito Ando, Abdul Rahim Raha, Kumar Sudesh, Hidayah Ariffin, Mohd Ali Hassan and Mohd Rafein Zakaria
  
  Recombinant Escherichia coli JM109 harbouring the polyhydroxyalkanoate (PHA) biosynthesis gene (phaCABco) of Comamonas sp. EB172, an acid tolerant microbe, was examined for the production of PHAs from various carbon sources. The study demonstrated that the recombinant E. coli JM109 had the potential to utilize both sugar- and acid-based carbon sources, for the biosynthesis of both poly(3-hydroxybutyrate) P(3HB) and poly(3- hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymers. In the shake flask experiments, the strain was capable of producing P (3HB-co-3HV) copolymer from mixed organic acids, and higher productivities were obtained using glucose compared to mixed acids. However, PHA accumulation was found to be similar, regardless of the carbon source used. Nitrogen sup.. View More»
  DOI: 10.4172/1948-5948.1000079

  Abstract PDF