Yinfeng Zhang
Tanzania
Research Article
Identification and Characterization of gtf, norB, and trx Genes in Flavobacterium Columnare
Author(s): Yinfeng Zhang and Covadonga R. AriasYinfeng Zhang and Covadonga R. Arias
Three genes from a shotgun genomic library of Flavobacterium columnare virulent strain ALG-00-530 were identified, characterized, and selected for differential expression analysis based on sequence similarity to putative virulence genes from related species. These genes were: glycosyltransferase (gtf), nitric oxide reductase (norB) and thioredoxin (trx). A collection of 30 F. columnare strains, including strains from genomovars I and II, were tested for the presence of these genes. Distribution patterns of gtf, norB, and trx across the species were not uniform. Nucleotide sequence variation was observed between genomovars for each gene; however, strains within the same genomovar shared identical gene sequences. Nine strains of F. columnare, from both genomovars, were chosen for gene expression analysis. The expression profile of these genes varied when selected strains were grown in v.. View More»
DOI:
10.4172/1948-5948.1000013