Journal of Microbial & Biochemical Technology

Victor Prima

Publications

• Rapid Communication
  Anti-LPS Test Strip for the Detection of Food Contaminated with Salmonella and E. coli
  Author(s): Alvin Wang, Gabriel Molina, Victor Prima and Kevin K.W. WangAlvin Wang, Gabriel Molina, Victor Prima and Kevin K.W. Wang
  
  Enterobacteria such as E. coli and Salmonella release an endotoxin, or lipopolysaccharide (LPS) that contributes to food poisoning symptoms. We hypothesize that LPS could be detected by a simple strip test, thus detecting the presence of enterobacteria in food. Here we described the development of a novel Anti-LPS detection strip test. Flexible polystyrene test strips with an absorbent poly (vinylidene fluoride) membrane adhered onto one end were exposed to various dilutions of food or bacterial sources. This was followed by the sequential incubation with a primary anti-LPS antibody, biotinylated secondary antibody, and streptavidin-linked enzyme alkaline phosphatase. The test strip was then developed with substrate 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium. Thus, the presence of purple precipitate product would indicate the presence of LPS and enterobacteria. The int.. View More»
  DOI: 10.4172/1948-5948.1000046

  Abstract PDF