Journal of Petroleum & Environmental Biotechnology

Stephen R Hughes

Publications

• Research Article
  Novel Antibacterial Polypeptide Laparaxin Produced by Lactobacillus paracasei Strain NRRL B-50314 Via Fermentation
  
  Author(s): Siqing Liu, Brian J Wilkinson, Kenneth M Bischoff, Stephen R Hughes, Joseph O Rich and Michael A CottaSiqing Liu, Brian J Wilkinson, Kenneth M Bischoff, Stephen R Hughes, Joseph O Rich and Michael A Cotta
  
  This study reports the production and characterization of a novel antibacterial polypeptide, designated laparaxin, which is secreted by Lactobacillus paracasei NRRL B-50314. Crude laparaxin has antibacterial activity against a wide variety of Gram-positive bacteria, including: lactic acid bacteria (Lactococcus lactis and Lactobacillus buchneri), food-borne pathogens (Listeria monocytogenes), gastrointestinal pathogens (Enterococcus faecalis), and opportunistic pathogens (Staphylococcus aureus methicillin-sensitive (MSSA) and methicillin resistant (MRSA) strains, a hetero-vancomycin-intermediate methicillin resistant strain (Hetero VISA also MRSA) MM66, and homogeneous vancomycin intermediate (Homo VISA). Using L. lactis as an indicator strain, the inhibitory activity of crude laparaxin was detected originally in early log phase, and the activity maximizes at the early stationary phase.. View More»
  DOI: 10.4172/2157-7463.1000121

  Abstract