Sita Kumari Karanam
Center for Biotechnology, Department of Chemical Engineering, College of Engineering, Andhra University, Visakhapatnam 530 003, AP,
India
Research Article
Application of Doehlert Experimental Design for the Optimization of Medium Constituents for the Production of L-asparaginase from Palm Kernal Cake (Elaeis guineensis)
Author(s): Sita Kumari Karanam and Narasimha Rao MedicherlaSita Kumari Karanam and Narasimha Rao Medicherla
Doehlert experimental design (DD) was applied for the optimization of medium constituents for L-asparaginase production by Yarrowia lipolytica NCIM 3472 in solid state fermentation (SSF) using palm kernel cake as the substrate. From the results of preliminary experimental runs, the three variables (glucose, moisture content, L-asparagine) have been identified as the potential variables for the production of L-asparaginase. Fifteen experimental runs designed by DD are carried out and the process response is modeled using a polynomial equation as function of these parameters. The proposed quadratic model for DD fitted very well to the experimental data that it could be used to navigate the design space according to analysis of variance results. The experimental values were in good agreement with predicted values and the correlation coefficient was found to be 0.9988. The optima.. View More»
DOI:
10.4172/1948-5948.1000016