Journal of Bacteriology & Parasitology

Shirzad Fallahi

Publications

• Research Article
  Development of Sensitive Nested Real-time PCR for Diagnosis of Acute and Chronic Phases of Toxoplasmosis in Mice Model
  Author(s): Parisa Mousavi, Hossein Mirhendi, Hossein Keshavarz Valian, Saeedeh Shojaee, Shirzad Fallahi, Armin Farhang, Mohammad-Ali Mohaghegh and Rasool JafariParisa Mousavi, Hossein Mirhendi, Hossein Keshavarz Valian, Saeedeh Shojaee, Shirzad Fallahi, Armin Farhang, Mohammad-Ali Mohaghegh and Rasool Jafari
  
  Toxoplasma gondii is an intracellular parasite that causes a variety of clinical manifestations. Acute and chronic phases of toxoplasmosis are considered as the presence of actively proliferating tachyzoites in the nucleated cells of mammalian hosts such as humans, and spread through blood to other parts of the body, which subsequently forms tissue cysts. The present study was aimed to evaluate the diagnostic value of nested real-time polymerase chain reaction (PCR) for the acute and chronic phases of toxoplasmosis in the laboratory mice using compared to conventional real-time PCR. To induce acute toxoplasmosis, 103 tachyzoites of Toxoplasma gondii RH strain were intraperitoneally inoculated to 25 BALB/c mice. In order to induce chronic toxoplasmosis, the mice were subcutaneously infected by the parasite and then treated with sulfadiazine from day one to day 14 post-injectio.. View More»
  DOI: 10.4172/2155-9597.1000341

  Abstract PDF