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Sharon A Huws
Sharon A Huws
Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais campus, Aberystwyth, SY23 3FG,
United Kingdom
Publications
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Research Article
Differential Colonization of Plant Parts by the Rumen Microbiota is likely to be due to Different Forage Chemistries
Author(s): Sharon A Huws, Olga L Mayorga, Michael K Theodorou, Eun J Kim, Alan H Cookson, Charles J Newbold and Alison H Kingston-SmithSharon A Huws, Olga L Mayorga, Michael K Theodorou, Eun J Kim, Alan H Cookson, Charles J Newbold and Alison H Kingston-Smith
In this experiment we investigated the hypothesis that heterogeneity of plant structures presents disparity in niches available for colonisation by the rumen microbiota resulting in differential colonisation. Fresh perennial ryegrass (PRG) stem and leaves were incubated in the presence of rumen bacteria under rumen-like conditions with incubations harvested at many time intervals up to 24 h. In vitro dry matter degradability (IVDMD) of stem material was lower than that of leaves at all harvesting times. Denaturing Gradient Gel Electrophoresis (DGGE) derived dendrograms, canonical analysis of principal coordinates (CAP) and PERMANOVA demonstrated that diversity of bacteria attached to PRG stem and leaf material was different at all harvesting times although QPCR data showed similar quantities of bacterial 16S rDNA on stem and leaf material at all harvesting times. Conversely, bacterial.. View More»
DOI: 10.4172/1948-5948.1000126