Using Translation Elongation Factor Gene to Specifically Detect and Diagnose Fusarium xylaroides, a Causative Agent of Coffee Wilt Disease in Ethiopia, East and Central Africa.
Author(s): Olal S, Olango N, Kiggundu A, Ochwo S, Adriko J, Nanteza A, Matovu E, Lubega GW, Kagezi G, Hakiza GJ, Wagoire WW, Rutherford MA and Opiyo SOOlal S, Olango N, Kiggundu A, Ochwo S, Adriko J, Nanteza A, Matovu E, Lubega GW, Kagezi G, Hakiza GJ, Wagoire WW, Rutherford MA and Opiyo SO
The present study presents the first report on the application of DNA-based polymerase chain reaction (PCR) for the specific detection and diagnosis of F usarium xylarioides (anamorph: G ibberrela xylarioides). Fusarium xylarioides is the causative agent of Coffee wilt disease (Tracheomycosis), and the disease is the most important economic constraint in Robusta coffee production in Uganda. The pathogen has two races, one pathogenic to Robusta coffee and the other to Arabica coffee, and not vice versa. Its laboratory diagnosis has been mainly based on microscopy, which is slow, has poor discriminative power, requires high expertise, only applicable on host plants with symptoms, and has since failed to detect the pathogen from the soil. Translation Elongation factor-1α (TEF-1α) gene from a F. xylarioides isolated from infected Robusta coffee plant was amplified by Fusarium .. Read More»