Roberto Fiorito
Italy
Research Article
Application of Real-Time PCR to Identify Residual Bio-Decontamination
of Confined Environments after Hydrogen Peroxide Vapor Treatment:
Preliminary Results
Author(s): Michele Pazienza, Maria Serena Britti, Mariachiara Carestia, Orlando Cenciarelli, Fabrizio D\'Amico, Andrea Malizia, Carlo Bellecci, Pasquale Gaudio, Antonio Gucciardino, Mariarosa Bellino, Corrado Lancia, Annalaura Tamburrini and Roberto FioritoMichele Pazienza, Maria Serena Britti, Mariachiara Carestia, Orlando Cenciarelli, Fabrizio D\'Amico, Andrea Malizia, Carlo Bellecci, Pasquale Gaudio, Antonio Gucciardino, Mariarosa Bellino, Corrado Lancia, Annalaura Tamburrini and Roberto Fiorito
This study was conducted to assess the effectiveness of Hydrogen Peroxide Vapor (HPV) to remove biological contamination in a confined environment and to evaluate real-time PCR assay as a technique for the evaluation of the decontamination efficiency. Decontamination after the dispersion of biological aerosol is a main issue from a civilian, public health and military perspective. Despite the effectiveness of aggressive substances, eco-friendly but still efficient methods for decontamination are a relevant demand and Hydrogen Peroxide Vapor (HPV) is among the most recent and promising technologies in this field. Another related issue is: when an environment can be considered fully decontaminated? The answer clearly depends on the objectives of the decontamination and this will affect the choice of the methodology. Furthermore, classical microbiological and molecular biology techniques.. View More»
DOI:
10.4172/1948-5948.1000116