Journal of Plant Pathology & Microbiology

RK Roohie

Publications

• Research Article
  Development of Multiplex PCR for the Specific Detection of Xanthomonas campestris pv. campestris in Cabbage and Correlation with Disease Incidence
  Author(s): RK Roohie and S UmeshaRK Roohie and S Umesha
  
  Black rot, the most serious disease of crucifers especially Brassica oleracea var. capitata (Cabbage) causes huge yield losses. Black rot is a seed-borne disease and the assessment of seeds for the pathogen is very crucial. The cabbage seeds were tested for X. campestris pv. campestris infection by biochemical and PCR analysis. The seed samples were subjected to PCR analysis with three primers, out of which one of the pairs of primers specific for hrpF gene could detect the black rot pathogen. The current study is the first of its kind wherein the locally available cabbage cultivars were assessed using molecular assays and the widely grown cultivars in the study area were checked for the correlation between laboratory and field performances. The aim of the study was to use the available specific and sensitive PCR-based procedures for routine detection of X. campestris pv. campestris i.. View More»
  DOI: 10.4172/2157-7471.1000127

  Abstract PDF