Journal of Stem Cell Research & Therapy

René van Weeren

Publications

• Research Article
  Laser Capture Microdissection of Murine Interzone Cells: Layer Selection and Prediction of RNA Yield
  Author(s): Florien Jenner, Gerjo JVM van Osch, Mairead Cleary, Iris Ribitsch, Ulrich Sauer, René van Weeren and Pieter BramaFlorien Jenner, Gerjo JVM van Osch, Mairead Cleary, Iris Ribitsch, Ulrich Sauer, René van Weeren and Pieter Brama
  
  Objective: Articular chondrocytes originate from a distinct cohort of progenitor cells located in the so-called interzone in embryonic developing joints. We conducted this study 1) to determine if it is possible to identify the intermediate and outer interzone layers histologically (using cresyl violet) without adjunct in situ hybridization or immunohistochemistry; 2) to establish whether sufficient amounts of RNA can be harvested from each interzone layer of individual embryos to allow gene expression analysis; 3) to develop measurements that can provide an estimation of the RNA yield prior to costly amplification steps. Methods: Cells from the outer (OI) and intermediate (II) interzone of the nascent femorotibial joint and the epiphyseal cartilage (EC) of femur and tibia of murine embryos of 13.5 and 15.5 days gestation were harvested using laser capture microdissection (LCM). Subse.. View More»
  DOI: 10.4172/2157-7633.1000183

  Abstract PDF