Clinical Microbiology: Open Access

Paul D Fey

Publications

• Research Article
  Methicillin Resistance and Biofilm Formation of Staphylococcus epidermidis in Blood Culture Isolates from Children under Five: A Multicenter Study in Nigeria
  Author(s): John-Ugwuanya A Grace, Busayo O Olayinka, Josiah A Onaolapo, Fatimah Hassan-Hanga, Huda Munir, Paul D Fey and Stephen K ObaroJohn-Ugwuanya A Grace, Busayo O Olayinka, Josiah A Onaolapo, Fatimah Hassan-Hanga, Huda Munir, Paul D Fey and Stephen K Obaro
  
  The clinical impact of Staphylococcus epidermidis in bacteremia remains controversial. The aim of this study is to determine the methicillin resistance and biofilm formation of S. epidermidis isolates. A total of 102 S. epidermidis blood culture isolates from children under five attending seven selected hospitals in north-central and north-west Nigeria within 2009 to 2016 were analyzed for methicillin resistance using cefoxitin disk agar diffusion test. Phenotypic biofilm formation and molecular detection of the intercellular adhesion locus (icaA) gene was performed by the quantitative Microtitre Plate (MTP) method and conventional Polymerase Chain Reaction (PCR) respectively. Seventy-four (72.5%) Methicillin-Resistant Staphylococcus epidermidis (MRSE) was observed while biofilm formation was detected in 20 (19.6%) S. epidermidis i.. View More»
  DOI: 10.4172/2327-5073.1000321

  Abstract PDF