Paola Di Donato
Italy
Research Article
Use of Agro Waste Biomass for α-Amylase Production by Anoxybacillus
amylolyticus: Purification and Properties
Author(s): Ilaria Finore, Paola Di Donato, Annarita Poli, Betul Kirdar, Ceyda Kasavi, Ebru O Toksoy, Barbara Nicolaus and Licia LamaIlaria Finore, Paola Di Donato, Annarita Poli, Betul Kirdar, Ceyda Kasavi, Ebru O Toksoy, Barbara Nicolaus and Licia Lama
Knowledge accumulated from fundamental and applied studies on Anoxybacillus suggests that this genus can serve as a good alternative in many applications related to starch and lignocellulosic biomasses, waste treatment, enzyme technology, and bioenergy production. We investigated the purification, biochemical characterization and immobilization of a thermostable α-amylase from the thermophilic Anoxybacillus amylolyticus, strain MR3CT, isolated in Antarctica and its production on vegetable wastes. In particular, the rhizome from Arundo donax, waste biomass of Cynara cardunculus and potato peels were tested either in Submerged Fermentation (SmF) and Solid State Fermentation (SSF) conditions. The amylase from A. amylolyticus, with a molecular weight of about 60 kDa, displayed an optimum enzyme activity at 60°C and pH 5.6. Moreover, by retaining up to 70% of total activity after.. View More»
DOI:
10.4172/1948-5948.1000162