Journal of Microbial & Biochemical Technology

Nageh Abo Dahab F

Publications

• Research Article
  Production, Purification and Characterization of L-Asparaginase from Marine Endophytic Aspergillus sp. ALAA-2000 under Submerged and Solid State Fermentation
  Author(s): Mervat Morsy Abbas Ahmed, Nageh Abo Dahab F, Taher Taha M and Fareed Hassan SMMervat Morsy Abbas Ahmed, Nageh Abo Dahab F, Taher Taha M and Fareed Hassan SM
  
  Among all endophytic fungi recovered from the marine soft sponge Aplysina fistularis, 72.2% were able to produce L-asparaginase. Among all obtained isolates, Aspergillus sp. ALAA-2000, the hyperactive producer for the anticancer agent, L-asparaginase, under submerged fermentation (SMF) and solid state fermentation (SSF) of different agriculture wastes was selected for the optimization of extraction process; optimization of physicochemical parameters, which affecting the production of L-asparaginases in SSF and optimization of the purified L-asparaginases parameters. Maximum L-asparaginase activity 23.34 U/ml was recovered from soybean with hot water at 40 °C and 150 rpm for 30 min under SSF and 30.64 U/ml under submerged fermentation using glucose as carbon source and asparagine as nitrogen source. Two types of L-asparaginase (AYA-1 and AYA-2) were pur.. View More»
  DOI: 10.4172/1948-5948.1000199

  Abstract PDF