Pharmaceutica Analytica Acta

Muhammad Yar Khuhawar

Publications

• Research Article
  HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples
  Author(s): Malik Alamgir, Muhammad Yar Khuhawar, Saima Qayoom Memon, Amir Hayat, Rizwan Ali Zounr and Asma ChanarMalik Alamgir, Muhammad Yar Khuhawar, Saima Qayoom Memon, Amir Hayat, Rizwan Ali Zounr and Asma Chanar
  
  A novel High Performance Liquid Chromatography (HPLC) method has been developed based on pre column derivatization with benzoin for determination of metformin, famotidine and ranitidine. The separation was achieved from C18 column when eluted isocratically, the solution of the drugs with methanol, water, acetonitrile and Tetra Hydrofuran (THF) (40:40:16:4 v/v) with a flow rate at 1 mL/min. UV detection was found to be 268 nm. Linear calibrations range was obtained with 2.5-12.5 ?g/ml, with limits of detection (LODs) 0.091-0.30 ?g/ml. The total run time for elution was 3.5 min. The derivitization, separation and quantitation was repeatable (n=4) in terms of retention time and peak height/peak area with Relative Standard Deviation (RSD) within 0.84-1.55% and 0.68-1.17% respectively. The method was applied for the analysis of metformin, famotidine and ranitidine from pharmaceutical preparat.. View More»
  DOI: 10.4172/2153-2435.1000546

  Abstract PDF